Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Disks large homolog 1 (DLG1) Standard Curve.)
Human Disks Large Homolog 1 (DLG1) ELISA Kit | DLG1 elisa kit
Human Disks Large Homolog 1 (DLG1) ELISA Kit
Gene Names
DLG1; hdlg; DLGH1; SAP97; SAP-97
Reactivity
Human
Synonyms
Disks Large Homolog 1 (DLG1); Human Disks Large Homolog 1 (DLG1) ELISA Kit; DLG1; DKFZp761P0818; DKFZp781B0426; DLGH1; SAP-97; SAP97; dJ1061C18.1.1; hdlg; OTTHUMP00000165203; discs large homolog 1; discs; large homolog 1; presynaptic protein SAP97; synapse-associated protein 97; DLG1 elisa kit
Reactivity
Human
Specificity
Human Disks large homolog 1 (DLG1) ELISA Kit has high sensitivity and excellent specificity for detection of Human DLG1. No significant cross-reactivity or interference between Human DLG1 and analogues was observed.
Sequence Length
904
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Disks large homolog 1 (DLG1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Disks large homolog 1 (DLG1) Standard Curve.)
Related Product Information for DLG1 elisa kit
Description: This Human Disks large homolog 1 (DLG1) ELISA Kit employs a two-site sandwich ELISA to quantitate DLG1 in samples. An antibody specific for DLG1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DLG1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DLG1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DLG1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: DLG7 is a kinetochore protein that stabilizes microtubules in vicinity of chromosomes. DLG7 controls spindle dynamics, promotes interkinetochore tension and efficient kinetochore capture. DGL7 is a part of Ran-dependent complex. Stabilization of DGL7 in cell occurs due to phosphorylation by Aurora A kinase. Expression of DGL7 is found in cancer and stem cells. The sequences of the remaining 20 genes were entirely new, and characteristic protein motifs or domains were identified in 32 genes. Other sequence features noted were that the coding sequences of 23 genes were followed by relatively long stretches of 3'-untranslated sequences and that 5 genes contained repetitive sequences in their 3'-untranslated regions. The chromosomal location of these genes has been determined.
Background: DLG7 is a kinetochore protein that stabilizes microtubules in vicinity of chromosomes. DLG7 controls spindle dynamics, promotes interkinetochore tension and efficient kinetochore capture. DGL7 is a part of Ran-dependent complex. Stabilization of DGL7 in cell occurs due to phosphorylation by Aurora A kinase. Expression of DGL7 is found in cancer and stem cells. The sequences of the remaining 20 genes were entirely new, and characteristic protein motifs or domains were identified in 32 genes. Other sequence features noted were that the coding sequences of 23 genes were followed by relatively long stretches of 3'-untranslated sequences and that 5 genes contained repetitive sequences in their 3'-untranslated regions. The chromosomal location of these genes has been determined.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
disks large homolog 1 isoform 1
NCBI Official Synonym Full Names
discs large MAGUK scaffold protein 1
NCBI Official Symbol
DLG1
NCBI Official Synonym Symbols
hdlg; DLGH1; SAP97; SAP-97
NCBI Protein Information
disks large homolog 1
UniProt Protein Name
Disks large homolog 1
Protein Family
UniProt Gene Name
DLG1
UniProt Synonym Gene Names
SAP-97; SAP97
UniProt Entry Name
DLG1_HUMAN
NCBI Description
This gene encodes a multi-domain scaffolding protein that is required for normal development. This protein may have a role in septate junction formation, signal transduction, cell proliferation, synaptogenesis and lymphocyte activation. A multitude of transcript variants deriving from alternative splicing and the use of multiple alternate promoter have been observed, including some splice variants that may be specific to brain and other tissues. An upstream uORF may regulate translation at some splice variants of this gene. [provided by RefSeq, Sep 2018]
Uniprot Description
Essential multidomain scaffolding protein required for normal development (). Recruits channels, receptors and signaling molecules to discrete plasma membrane domains in polarized cells. May play a role in adherens junction assembly, signal transduction, cell proliferation, synaptogenesis and lymphocyte activation. Regulates the excitability of cardiac myocytes by modulating the functional expression of Kv4 channels. Functional regulator of Kv1.5 channel.
Research Articles on DLG1
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Product Notes
The Human DLG1 dlg1 (Catalog #AAA9715693) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715693 ELISA Kit recognizes Human DLG1. It is sometimes possible for the material contained within the vial of "Disks Large Homolog 1 (DLG1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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