Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Dihydropteridine reductase (QDPR) Standard Curve.)
Human Dihydropteridine Reductase (QDPR) ELISA Kit | QDPR elisa kit
Human Dihydropteridine Reductase (QDPR) ELISA Kit
Gene Names
QDPR; DHPR; PKU2; HDHPR; SDR33C1
Reactivity
Human
Synonyms
Dihydropteridine Reductase (QDPR); Human Dihydropteridine Reductase (QDPR) ELISA Kit; QDPR; DHPR; FLJ42391; PKU2; SDR33C1; 6; 7-dihydropteridine reductase; short chain dehydrogenase/reductase family 33C; member 1; QDPR elisa kit
Reactivity
Human
Specificity
Human Dihydropteridine reductase (QDPR) ELISA Kit has high sensitivity and excellent specificity for detection of Human QDPR. No significant cross-reactivity or interference between Human QDPR and analogues was observed.
Sequence Length
244
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Dihydropteridine reductase (QDPR) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Dihydropteridine reductase (QDPR) Standard Curve.)
Related Product Information for QDPR elisa kit
Description: This Human Dihydropteridine reductase (QDPR) ELISA Kit employs a two-site sandwich ELISA to quantitate QDPR in samples. An antibody specific for QDPR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any QDPR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for QDPR is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of QDPR bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: QDPR (quinoid dihydropteridine reductase) is part of the pathway that recycles a substance called tetrahydrobiopterin, also known as BH4. Tetrahydrobiopterin works with an enzyme called phenylalanine hydroxylase to process a substance called phenylalanine. Phenylalanine is an amino acid (a building block of proteins) that is obtained through the diet; it is found in all proteins and in some artificial sweeteners. When tetrahydrobiopterin interacts with phenylalanine hydroxylase, tetrahydrobiopterin is altered and must be recycled to a usable form. The regeneration of this substance is critical for the proper processing of several other amino acids in the body. Tetrahydrobiopterin also helps produce certain chemicals in the brain called neurotransmitters, which transmit signals between nerve cells.
Background: QDPR (quinoid dihydropteridine reductase) is part of the pathway that recycles a substance called tetrahydrobiopterin, also known as BH4. Tetrahydrobiopterin works with an enzyme called phenylalanine hydroxylase to process a substance called phenylalanine. Phenylalanine is an amino acid (a building block of proteins) that is obtained through the diet; it is found in all proteins and in some artificial sweeteners. When tetrahydrobiopterin interacts with phenylalanine hydroxylase, tetrahydrobiopterin is altered and must be recycled to a usable form. The regeneration of this substance is critical for the proper processing of several other amino acids in the body. Tetrahydrobiopterin also helps produce certain chemicals in the brain called neurotransmitters, which transmit signals between nerve cells.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
dihydropteridine reductase isoform 1
NCBI Official Synonym Full Names
quinoid dihydropteridine reductase
NCBI Official Symbol
QDPR
NCBI Official Synonym Symbols
DHPR; PKU2; HDHPR; SDR33C1
NCBI Protein Information
dihydropteridine reductase
UniProt Protein Name
Dihydropteridine reductase
Protein Family
UniProt Gene Name
QDPR
UniProt Synonym Gene Names
DHPR
UniProt Entry Name
DHPR_HUMAN
NCBI Description
This gene encodes the enzyme dihydropteridine reductase, which catalyzes the NADH-mediated reduction of quinonoid dihydrobiopterin. This enzyme is an essential component of the pterin-dependent aromatic amino acid hydroxylating systems. Mutations in this gene resulting in QDPR deficiency include aberrant splicing, amino acid substitutions, insertions, or premature terminations. Dihydropteridine reductase deficiency presents as atypical phenylketonuria due to insufficient production of biopterin, a cofactor for phenylalanine hydroxylase. [provided by RefSeq, Jul 2008]
Research Articles on QDPR
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Product Notes
The Human QDPR qdpr (Catalog #AAA9714672) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714672 ELISA Kit recognizes Human QDPR. It is sometimes possible for the material contained within the vial of "Dihydropteridine Reductase (QDPR), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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