Typical Testing Data/Standard Curve (for reference only)
Human DHEA-S ELISA Kit | DHEA-S elisa kit
DHEA-S ELISA Kit
Reactivity
Human
Synonyms
DHEA-S; DHEA-S ELISA Kit; DHEA-S elisa kit
Reactivity
Human
Samples
Serum and plasma
Assay Type
Quantitative Competitive
Preparation and Storage
Kit should be stored at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for DHEA-S elisa kit
Intended Uses: DHEA-S ELISA kit is used for the quantitative measurement of DHEAS in human serum and plasma.
Principle of the Assay: DHEA-S kit is based on the principle of competitive binding between DHAEA-S in the test specimen and HRP conjugated DHEA-S for a constant amount of anti-DHEA-S antibody. In the first incubation, Goat-anti-Rabbit-lgG coated wells are incubated with 1 O~I of DHEA-S standards, patient samples, 50~1 DHEA-S Enzyme (HRP) reagent and 50~1 anti-DHEA-S Antibody reagent, at room temperature, for 60 minutes. During the incubation, HRP labeled DHEA-S competes with the endogenous DHEA-S in the standard and sample, for a fixed number of binding sites of the DHEA-S antibody, while simultaneously the Anti DHEA-S antibody binds to the immobilized secondary antibody. Thus, the amount of DHEA-S HRP conjugate immunologically bound to the well progressively decreases as the concentration of DHEA-S in the specimen increases. Unbound DHEA-S HRP conjugate is then removed and the wells washed. Next, TMB Reagent is added and incubated at room temperature for 30 minutes, resulting in the development of blue color. The color development is stopped with the addition of stop solution, and the absorbance is spectrophotometrically measured at 450nm. A standard curve is prepared relating color intensity to the concentration of DHEA-S.
Principle of the Assay: DHEA-S kit is based on the principle of competitive binding between DHAEA-S in the test specimen and HRP conjugated DHEA-S for a constant amount of anti-DHEA-S antibody. In the first incubation, Goat-anti-Rabbit-lgG coated wells are incubated with 1 O~I of DHEA-S standards, patient samples, 50~1 DHEA-S Enzyme (HRP) reagent and 50~1 anti-DHEA-S Antibody reagent, at room temperature, for 60 minutes. During the incubation, HRP labeled DHEA-S competes with the endogenous DHEA-S in the standard and sample, for a fixed number of binding sites of the DHEA-S antibody, while simultaneously the Anti DHEA-S antibody binds to the immobilized secondary antibody. Thus, the amount of DHEA-S HRP conjugate immunologically bound to the well progressively decreases as the concentration of DHEA-S in the specimen increases. Unbound DHEA-S HRP conjugate is then removed and the wells washed. Next, TMB Reagent is added and incubated at room temperature for 30 minutes, resulting in the development of blue color. The color development is stopped with the addition of stop solution, and the absorbance is spectrophotometrically measured at 450nm. A standard curve is prepared relating color intensity to the concentration of DHEA-S.
Product Categories/Family for DHEA-S elisa kit
Similar Products
Product Notes
The Human DHEA-S (Catalog #AAA580169) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA580169 ELISA Kit recognizes Human DHEA-S. It is sometimes possible for the material contained within the vial of "DHEA-S, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
