Human Dermcidin (DCD) ELISA Kit | DCD elisa kit
Human Dermcidin (DCD) ELISA Kit
Gene Names
DCD; PIF; AIDD; DSEP; HCAP; DCD-1
Reactivity
Human
Synonyms
Dermcidin (DCD); Human Dermcidin (DCD) ELISA Kit; HCAP; AIDD; DCD1; DSEP; PIF; Proteolysis Inducing Factor; Preproteolysin; Diffusible Survival/Evasion Peptide; Survival Promoting Peptide; DCD elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of dermcidin. No significant cross-reactivity or interference between dermcidin and analogues was observed.
Sequence Length
77
Samples
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
0.78-50ng/mL
Sensitivity
< 0.29ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level dermcidin were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level dermcidin were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for DCD elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of dermcidin in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to dermcidin. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to dermcidin. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain dermcidin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of dermcidin in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to dermcidin. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to dermcidin. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain dermcidin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of dermcidin in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
8,295 Da
NCBI Official Full Name
dermcidin isoform 1 preproprotein
NCBI Official Synonym Full Names
dermcidin
NCBI Official Symbol
DCD
NCBI Official Synonym Symbols
PIF; AIDD; DSEP; HCAP; DCD-1
NCBI Protein Information
dermcidin
UniProt Protein Name
Dermcidin
Protein Family
UniProt Gene Name
DCD
UniProt Synonym Gene Names
AIDD; DSEP
NCBI Description
This antimicrobial gene encodes a secreted protein that is subsequently processed into mature peptides of distinct biological activities. The C-terminal peptide is constitutively expressed in sweat and has antibacterial and antifungal activities. The N-terminal peptide, also known as diffusible survival evasion peptide, promotes neural cell survival under conditions of severe oxidative stress. A glycosylated form of the N-terminal peptide may be associated with cachexia (muscle wasting) in cancer patients. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2014]
Uniprot Description
DCD-1 displays antimicrobial activity thereby limiting skin infection by potential pathogens in the first few hours after bacterial colonization. Highly effective against E.coli, E.faecalis, S.aureus and C.albicans. Optimal pH and salt concentration resemble the conditions in sweat. Also exhibits proteolytic activity, cleaving on the C-terminal side of Arg and, to a lesser extent, Lys residues (PubMed:17448443).
