Human Dedicator of cytokinesis protein 8 (DOCK8) ELISA Kit | DOCK8 elisa kit

Human Dedicator of cytokinesis protein 8 (DOCK8) ELISA Kit

Cat. Num. AAA2802230

• Gene Names: DOCK8; MRD2; ZIR8; HEL-205
• Reactivity: Human
• Synonyms: Dedicator of cytokinesis protein 8 (DOCK8); Human Dedicator of cytokinesis protein 8 (DOCK8) ELISA Kit; RP11-165F24.7; FLJ00026; FLJ00152; FLJ00346; MRD2; ZIR8; 1200017A24Rik; DOCK8 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human DOCK8. No significant cross-reactivity or interference between Human DOCK8 and analogues was observed.

• Assay Type: Sandwich
• Assay: Serum, Plasma, Other biological fluids
• Analysis Method: Quantitive
• Detection Wavelength: 450nm
• Sample Volume: 1-200uL
• Assay Duration: 1-4.5h
• Precision: Intra-assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Test Principle: This assay employs a two-site sandwich ELISA to quantitate DOCK8 in samples. An antibody specific for DOCK8 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDOCK8 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DOCK8 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DOCK8 bound in the initial step. The color development is stopped and the intensity of the color is measured.
• Preparation and Storage: Store at 2-8 degree C. Please refer to Instruction Manual.

Related Product Information for DOCK8 elisa kit

Dock8 was identified during a yeast two hybrid (YTH) screen for proteins that interact with the Rho family small G protein Cdc42.Subsequent northern blot analysis revealed high levels of Dock8 expression in the placenta, lung, kidney and pancreas as well as lower levels in the heart, brain and skeletal muscle.
Dock8 shares the same core domain arrangement as all other DOCK proteins, with a DHR2 domain which, in other proteins, contains GEF activity and a DHR1 domain known, in other proteins, to interact with phospholipids. In the YTH system Dock8 was reported to interact with both Rac1 and Cdc42. However, no stable interaction between Dock8 and these small G proteins was observed in a GST-pulldown assay.

NCBI and Uniprot Product Information

• NCBI GI #: 302129691
• NCBI GeneID: 81704
• NCBI Accession #: NP_001180465.1
• NCBI GenBank Nucleotide #: NM_001193536.1
• UniProt Accession #: Q8NF50; Q3MV16; Q5JPJ1; Q8TEP1; Q8WUY2; Q9BYJ5; Q9H1Q2; A2A350; A2BDF2; A4FU78; B7ZLP0; E9PH09
• Molecular Weight: 238,529 Da
• NCBI Official Full Name: dedicator of cytokinesis protein 8 isoform 3
• NCBI Official Synonym Full Names: dedicator of cytokinesis 8
• NCBI Official Symbol: DOCK8
• NCBI Official Synonym Symbols: MRD2; ZIR8; HEL-205
• NCBI Protein Information: dedicator of cytokinesis protein 8; 1200017A24Rik; epididymis luminal protein 205
• UniProt Protein Name: Dedicator of cytokinesis protein 8
• Protein Family: Dedicator of cytokinesis protein
• UniProt Gene Name: DOCK8
• UniProt Entry Name: DOCK8_HUMAN

NCBI Description

This gene encodes a member of the DOCK180 family of guanine nucleotide exchange factors. Guanine nucleotide exchange factors interact with Rho GTPases and are components of intracellular signaling networks. Mutations in this gene result in the autosomal recessive form of the hyper-IgE syndrome. Alternatively spliced transcript variants encoding different isoforms have been described.[provided by RefSeq, Jun 2010]

Uniprot Description

DOCK8: Potential guanine nucleotide exchange factor (GEF). GEF proteins activate some small GTPases by exchanging bound GDP for free GTP. Defects in DOCK8 are the cause of hyperimmunoglobulin E recurrent infection syndrome autosomal recessive (AR-HIES). It is a rare disorder of immunity characterized by immunodeficiency, recurrent infections, eczema, increased serum IgE, eosinophilia and lack of connective tissue and skeletal involvement. Defects in DOCK8 are the cause of mental retardation autosomal dominant type 2 (MRD2). Mental retardation is characterized by significantly below average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. A chromosomal aberration disrupting DOCK8 has been found in a patient with mental retardation and ectodermal dysplasia. DOCK8 is disrupted in patients with mental retardation. A balanced translocation, t(X;9) (q13.1;p24). Belongs to the DOCK family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: GEFs; GEFs, misc.; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 9p24.3

Cellular Component: membrane; leading edge; cytosol

Molecular Function: protein binding; guanyl-nucleotide exchange factor activity

Biological Process: formation of immunological synapse; small GTPase mediated signal transduction; blood coagulation; positive regulation of GTPase activity

Disease: Mental Retardation, Autosomal Dominant 2; Hyper-ige Recurrent Infection Syndrome, Autosomal Recessive