Human D-amino Acid Oxidase Activator (DAOA) ELISA Kit | DAOA elisa kit

Human D-amino Acid Oxidase Activator (DAOA) ELISA Kit

Cat. Num. AAA451334

• Gene Names: DAOA; LG72; SG72
• Reactivity: Human
• Synonyms: D-amino Acid Oxidase Activator (DAOA); Human D-amino Acid Oxidase Activator (DAOA) ELISA Kit; G72; LG72; SG72; DAOA elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of DAOA. No significant cross-reactivity or interference between DAOA and analogues was observed.
• Sequence Length: 125

• Samples: Tissue homogenates, cell lysates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.061ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level DAOA were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level DAOA were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for DAOA elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of DAOA in human tissue homogenates, cell lysates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to DAOA. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to DAOA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain DAOA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of DAOA in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 240120173
• NCBI GeneID: 267012
• NCBI Accession #: NP_001155284.1
• NCBI GenBank Nucleotide #: NM_001161812.1
• UniProt Accession #: P59103; Q0VAE6; Q5VX59; Q86Y17; Q8IWM4; A6NKG7
• Molecular Weight: 14,719 Da
• NCBI Official Full Name: D-amino acid oxidase activator isoform 2
• NCBI Official Synonym Full Names: D-amino acid oxidase activator
• NCBI Official Symbol: DAOA
• NCBI Official Synonym Symbols: LG72; SG72
• NCBI Protein Information: D-amino acid oxidase activator
• UniProt Protein Name: D-amino acid oxidase activator
• Protein Family: D-amino acid oxidase activator
• UniProt Gene Name: DAOA
• UniProt Synonym Gene Names: G72

NCBI Description

This gene encodes a protein that may function as an activator of D-amino acid oxidase, which degrades the gliotransmitter D-serine, a potent activator of N-methyl-D-aspartate (NMDA) type glutamate receptors. Studies also suggest that one encoded isoform may play a role in mitochondrial function and dendritic arborization. Polymorphisms in this gene have been implicated in susceptibility to schizophrenia and bipolar affective disorder. Alternatively spliced transcript variants encoding different isoforms have been identified.[provided by RefSeq, Mar 2011]

Uniprot Description

Seems to activate D-amino acid oxidase.