Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Cytoplasmic dynein 2 heavy chain 1 (DYNC2H1) Standard Curve.)
Human Cytoplasmic Dynein 2 Heavy Chain 1 (DYNC2H1) ELISA Kit | DYNC2H1 elisa kit
Human Cytoplasmic Dynein 2 Heavy Chain 1 (DYNC2H1) ELISA Kit
Gene Names
DYNC2H1; ATD3; DHC2; DHC1b; DNCH2; DYH1B; SRTD3; SRPS2B; hdhc11
Reactivity
Human
Synonyms
Cytoplasmic Dynein 2 Heavy Chain 1 (DYNC2H1); Human Cytoplasmic Dynein 2 Heavy Chain 1 (DYNC2H1) ELISA Kit; DYNC2H1; ATD3; DHC1b; DHC2; DNCH2; DYH1B; FLJ11756; hdhc11; DYNC2H1 variant protein; cytoplasmic dynein 2 heavy chain; dynein heavy chain; isotype 1B; dynein; cytoplasmic; heavy chain-2; hea; DYNC2H1 elisa kit
Reactivity
Human
Specificity
Human Cytoplasmic dynein 2 heavy chain 1 (DYNC2H1) ELISA Kit has high sensitivity and excellent specificity for detection of Human DYNC2H1. No significant cross-reactivity or interference between Human DYNC2H1 and analogues was observed.
Sequence Length
4307
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Cytoplasmic dynein 2 heavy chain 1 (DYNC2H1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Cytoplasmic dynein 2 heavy chain 1 (DYNC2H1) Standard Curve.)
Related Product Information for DYNC2H1 elisa kit
Description: This Human Cytoplasmic dynein 2 heavy chain 1 (DYNC2H1) ELISA Kit employs a two-site sandwich ELISA to quantitate DYNC2H1 in samples. An antibody specific for DYNC2H1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DYNC2H1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DYNC2H1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DYNC2H1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: DYNC2H1 encodes a large cytoplasmic dynein protein that is involved in retrograde transport in the cilium and has a role in intraflagellar transport, a process required for ciliary/flagellar assembly. Mutations in this gene cause a heterogeneous spectrum of conditions related to altered primary cilium function and often involve polydactyly, abnormal skeletogenesis, and polycystic kidneys. Alternative splicing results in multiple transcript variants encoding distinct proteins. The light intermediate chains, with molecular masses of 55 to 59 kD, are unique to cytoplasmic dynein and contain consensus ATPase elements. Both axonemal and cytoplasmic dyneins also contain light chains of 8 to 25 kD.
Background: DYNC2H1 encodes a large cytoplasmic dynein protein that is involved in retrograde transport in the cilium and has a role in intraflagellar transport, a process required for ciliary/flagellar assembly. Mutations in this gene cause a heterogeneous spectrum of conditions related to altered primary cilium function and often involve polydactyly, abnormal skeletogenesis, and polycystic kidneys. Alternative splicing results in multiple transcript variants encoding distinct proteins. The light intermediate chains, with molecular masses of 55 to 59 kD, are unique to cytoplasmic dynein and contain consensus ATPase elements. Both axonemal and cytoplasmic dyneins also contain light chains of 8 to 25 kD.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
cytoplasmic dynein 2 heavy chain 1 isoform 1
NCBI Official Synonym Full Names
dynein cytoplasmic 2 heavy chain 1
NCBI Official Symbol
DYNC2H1
NCBI Official Synonym Symbols
ATD3; DHC2; DHC1b; DNCH2; DYH1B; SRTD3; SRPS2B; hdhc11
NCBI Protein Information
cytoplasmic dynein 2 heavy chain 1
UniProt Protein Name
Cytoplasmic dynein 2 heavy chain 1
UniProt Gene Name
DYNC2H1
UniProt Synonym Gene Names
DHC1B; DHC2; DNCH2; DYH1B; KIAA1997; hDHC11
NCBI Description
This gene encodes a large cytoplasmic dynein protein that is involved in retrograde transport in the cilium and has a role in intraflagellar transport, a process required for ciliary/flagellar assembly. Mutations in this gene cause a heterogeneous spectrum of conditions related to altered primary cilium function and often involve polydactyly, abnormal skeletogenesis, and polycystic kidneys. Alternative splicing results in multiple transcript variants encoding distinct proteins. [provided by RefSeq, Jan 2010]
Uniprot Description
May function as a motor for intraflagellar retrograde transport. Functions in cilia biogenesis. May play a role in transport between endoplasmic reticulum and Golgi or organization of the Golgi in cells ().
Research Articles on DYNC2H1
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Product Notes
The Human DYNC2H1 dync2h1 (Catalog #AAA9715627) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715627 ELISA Kit recognizes Human DYNC2H1. It is sometimes possible for the material contained within the vial of "Cytoplasmic Dynein 2 Heavy Chain 1 (DYNC2H1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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