Human Cytoplasmic dynein 1 light intermediate chain 2 (DYNC1LI2) ELISA Kit | DYNC1LI2 elisa kit

Human Cytoplasmic dynein 1 light intermediate chain 2 (DYNC1LI2) ELISA Kit

Cat. Num. AAA7248160

• Gene Names: DYNC1LI2; LIC2; DNCLI2
• Reactivity: Human
• Synonyms: Cytoplasmic dynein 1 light intermediate chain 2 (DYNC1LI2); Human Cytoplasmic dynein 1 light intermediate chain 2 (DYNC1LI2) ELISA Kit; DYNC1LI2 elisa kit

• Reactivity: Human

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 0.5-10ng/mL
• Sensitivity: 0.1ng/mL
• Intended Uses: This DYNC1LI2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human DYNC1LI2. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for DYNC1LI2 elisa kit

Principle of the assay: DYNC1LI2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-DYNC1LI2 antibody and an DYNC1LI2-HRP conjugate. The assay sample and buffer are incubated together with DYNC1LI2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the DYNC1LI2 concentration since DYNC1LI2 from samples and DYNC1LI2-HRP conjugate compete for the anti-DYNC1LI2 antibody binding site. Since the number of sites is limited, as more sites are occupied by DYNC1LI2 from the sample, fewer sites are left to bind DYNC1LI2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DYNC1LI2 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for DYNC1LI2 elisa kit

Neurobiology

NCBI and Uniprot Product Information

• NCBI GI #: 5453634
• NCBI GeneID: 1783
• NCBI Accession #: NP_006132.1
• NCBI GenBank Nucleotide #: NM_006141.2
• Molecular Weight: 45,005 Da
• NCBI Official Full Name: cytoplasmic dynein 1 light intermediate chain 2 isoform 1
• NCBI Official Synonym Full Names: dynein, cytoplasmic 1, light intermediate chain 2
• NCBI Official Symbol: DYNC1LI2
• NCBI Official Synonym Symbols: LIC2; DNCLI2
• NCBI Protein Information: cytoplasmic dynein 1 light intermediate chain 2; LIC-2; LIC53/55; dynein light intermediate chain 2, cytosolic; dynein, cytoplasmic, light intermediate polypeptide 2
• UniProt Protein Name: Cytoplasmic dynein 1 light intermediate chain 2
• Protein Family: Cytoplasmic dynein
• UniProt Gene Name: DYNC1LI2
• UniProt Synonym Gene Names: DNCLI2; LIC2; LIC-2
• UniProt Entry Name: DC1L2_HUMAN

NCBI Description

Cytoplasmic dynein is a microtubule-associated motor protein (Hughes et al., 1995 [PubMed 7738094]). See DYNC1H1 (MIM 600112) for general information about dyneins.[supplied by OMIM, Mar 2008]

Uniprot Description

DNCLI2: Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. May play a role in binding dynein to membranous organelles or chromosomes. Belongs to the dynein light intermediate chain family.

Protein type: Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 16q22.1

Cellular Component: centrosome; microtubule; membrane; cytoplasmic dynein complex; cytosol

Molecular Function: microtubule motor activity; ATP binding

Biological Process: centrosome localization; transport; metabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class II; microtubule cytoskeleton organization and biogenesis; microtubule-based movement