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Typical Testing Data/Standard Curve (for reference only) (Fig.1. Human Cytochrome P450 2U1 (CYP2U1) Standard Curve.)

Human Cytochrome P450 2U1 (CYP2U1) ELISA Kit | CYP2U1 elisa kit

Human Cytochrome P450 2U1 (CYP2U1) ELISA Kit

Gene Names
CYP2U1; SPG49; SPG56; P450TEC
Reactivity
Human
Synonyms
Cytochrome P450 2U1 (CYP2U1); Human Cytochrome P450 2U1 (CYP2U1) ELISA Kit; CYP2U1; P450TEC; CYP2U1 elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Specificity
Human Cytochrome P450 2U1 (CYP2U1) ELISA Kit has high sensitivity and excellent specificity for detection of Human CYP2U1. No significant cross-reactivity or interference between Human CYP2U1 and analogues was observed.
Sequence Length
544
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Human Cytochrome P450 2U1 (CYP2U1) Standard Curve.)

Typical Testing Data/Standard Curve (for reference only) (Fig.1. Human Cytochrome P450 2U1 (CYP2U1) Standard Curve.)
Related Product Information for CYP2U1 elisa kit
Description: This Human Cytochrome P450 2U1 (CYP2U1) ELISA Kit employs a two-site sandwich ELISA to quantitate CYP2U1 in samples. An antibody specific for CYP2U1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CYP2U1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CYP2U1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CYP2U1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: CYP2U1 encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This enzyme is a hydroxylase that metabolizes arachidonic acid, docosahexaenoic acid, and other long chain fatty acids.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
cytochrome P450 2U1
NCBI Official Synonym Full Names
cytochrome P450 family 2 subfamily U member 1
NCBI Official Symbol
CYP2U1
NCBI Official Synonym Symbols
SPG49; SPG56; P450TEC
NCBI Protein Information
cytochrome P450 2U1
UniProt Protein Name
Cytochrome P450 2U1
Protein Family
UniProt Gene Name
CYP2U1
UniProt Entry Name
CP2U1_HUMAN

NCBI Description

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This enzyme is a hydroxylase that metabolizes arachidonic acid, docosahexaenoic acid, and other long chain fatty acids. [provided by RefSeq, Jul 2008]

Uniprot Description

CYP2U1: Catalyzes the hydroxylation of arachidonic acid, docosahexaenoic acid and other long chain fatty acids. May modulate the arachidonic acid signaling pathway and play a role in other fatty acid signaling processes. Belongs to the cytochrome P450 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Oxidoreductase; EC 1.14.14.1; Membrane protein, integral; Lipid Metabolism - arachidonic acid; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 4q25

Cellular Component: endoplasmic reticulum membrane; intracellular membrane-bound organelle; cytoplasm; integral to membrane

Molecular Function: iron ion binding; heme binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen; steroid hydroxylase activity; oxygen binding

Biological Process: organic acid metabolic process; xenobiotic metabolic process; exogenous drug catabolic process; arachidonic acid metabolic process

Disease: Spastic Paraplegia 56, Autosomal Recessive

Research Articles on CYP2U1

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Product Notes

The Human CYP2U1 cyp2u1 (Catalog #AAA9715825) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715825 ELISA Kit recognizes Human CYP2U1. It is sometimes possible for the material contained within the vial of "Cytochrome P450 2U1 (CYP2U1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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