Human Cytochrome P450 26A1 (CYP26A1) ELISA Kit | CYP26A1 elisa kit

Human Cytochrome P450 26A1 (CYP26A1) ELISA Kit

Cat. Num. AAA453829

• Gene Names: CYP26A1; CP26; CYP26; P450RAI; P450RAI1
• Reactivity: Human
• Synonyms: Cytochrome P450 26A1 (CYP26A1); Human Cytochrome P450 26A1 (CYP26A1) ELISA Kit; CP26; CYP26; P450RAI; P450RAI1; hP450RAI; Cytochrome P450 retinoic acid-inactivating 1; Retinoic acid 4-hydroxylase; Retinoic acid-metabolizing cytochrome; CYP26A1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of CYP26A1. No significant cross-reactivity or interference between CYP26A1 and analogues was observed.
• Sequence Length: 497

• Samples: Tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.055ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CYP26A1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CYP26A1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CYP26A1 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CYP26A1 in human tissue homogenates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CYP26A1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to CYP26A1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CYP26A1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of CYP26A1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 16933530
• NCBI GeneID: 1592
• NCBI Accession #: NP_000774.2
• NCBI GenBank Nucleotide #: NM_000783.3
• UniProt Accession #: O43174; Q5VXH9; Q5VXI0; B3KNI4
• Molecular Weight: 48,558 Da
• NCBI Official Full Name: cytochrome P450 26A1 isoform 1
• NCBI Official Synonym Full Names: cytochrome P450 family 26 subfamily A member 1
• NCBI Official Symbol: CYP26A1
• NCBI Official Synonym Symbols: CP26; CYP26; P450RAI; P450RAI1
• NCBI Protein Information: cytochrome P450 26A1
• UniProt Protein Name: Cytochrome P450 26A1
• Protein Family: Cytochrome
• UniProt Gene Name: CYP26A1
• UniProt Synonym Gene Names: CYP26; P450RAI1; Cytochrome P450RAI; hP450RAI

NCBI Description

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum protein acts on retinoids, including all-trans-retinoic acid (RA), with both 4-hydroxylation and 18-hydroxylation activities. This enzyme regulates the cellular level of retinoic acid which is involved in regulation of gene expression in both embryonic and adult tissues. Two alternatively spliced transcript variants of this gene, which encode the distinct isoforms, have been reported. [provided by RefSeq, Jul 2008]

Uniprot Description

Plays a key role in retinoic acid metabolism. Acts on retinoids, including all-trans-retinoic acid (RA) and its stereoisomer 9-cis-RA. Capable of both 4-hydroxylation and 18-hydroxylation. Responsible for generation of several hydroxylated forms of RA, including 4-OH-RA, 4-oxo-RA and 18-OH-RA.