Human Cysteine protease ATG4B (ATG4B) ELISA Kit | ATG4B elisa kit

Human Cysteine protease ATG4B (ATG4B) ELISA Kit

Cat. Num. AAA7226180

• Gene Names: ATG4B; APG4B; AUTL1
• Reactivity: Human
• Synonyms: Cysteine protease ATG4B (ATG4B); Human Cysteine protease ATG4B (ATG4B) ELISA Kit; ATG4B elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of ATG4B. No significant cross-reactivity or interference between ATG4B and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ATG4B and all the analogues, therefore, cross reaction may still exist in some cases.

• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Assay Type: Quantitative Competitive
• Sensitivity: 0.1 ng/mL
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ATG4B elisa kit

Intended Uses: This ATG4B ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ATG4B. This ELISA kit for research use only, not for therapeutic or test applications!

Principle of the Assay: ATG4B ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-ATG4B antibody and an ATG4B-HRP conjugate. The assay sample and buffer are incubated together with ATG4B-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ATG4B concentration since ATG4B from samples and ATG4B-HRP conjugate compete for the anti-ATG4B antibody binding site. Since the number of sites is limited, as more sites are occupied by ATG4B from the sample, fewer sites are left to bind ATG4B-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ATG4B concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 47132611
• NCBI GeneID: 23192
• NCBI Accession #: NP_037457.3
• NCBI GenBank Nucleotide #: NM_013325.4
• Molecular Weight: 42,622 Da
• NCBI Official Full Name: cysteine protease ATG4B isoform a
• NCBI Official Synonym Full Names: autophagy related 4B, cysteine peptidase
• NCBI Official Symbol: ATG4B
• NCBI Official Synonym Symbols: APG4B; AUTL1
• NCBI Protein Information: cysteine protease ATG4B; hAPG4B; autophagin-1; APG4 autophagy 4 homolog B; AUT-like 1 cysteine endopeptidase; ATG4 autophagy related 4 homolog B; autophagy-related protein 4 homolog B; autophagy-related cysteine endopeptidase 1
• UniProt Protein Name: Cysteine protease ATG4B
• Protein Family: Cysteine protease
• UniProt Gene Name: ATG4B
• UniProt Synonym Gene Names: APG4B; AUTL1; KIAA0943; hAPG4B
• UniProt Entry Name: ATG4B_HUMAN

NCBI Description

Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

ATG4B: Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. Mainly expressed in the skeletal muscle, followed by brain, heart, liver and pancreas. Inhibited by N-ethylmaleimide. Belongs to the peptidase C54 family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Autophagy; Protease; Ubiquitin conjugating system; EC 3.4.22.-

Chromosomal Location of Human Ortholog: 2q37.3

Cellular Component: cytoplasm; cytosol

Molecular Function: protein binding; cysteine-type endopeptidase activity; endopeptidase activity; cysteine-type peptidase activity

Biological Process: protein delipidation; positive regulation of protein catabolic process; mitochondrion degradation; autophagy; protein processing; C-terminal protein lipidation; protein targeting to membrane; proteolysis; positive regulation of autophagy; autophagic vacuole formation; cellular response to nitrogen starvation