Human Cyclophilin D (CYPD) ELISA Kit | CYPD elisa kit

Human Cyclophilin D (CYPD) ELISA Kit

Cat. Num. AAA2097963

• Gene Names: PPID; CYPD; CYP-40
• Reactivity: Human
• Synonyms: Cyclophilin D (CYPD); Human Cyclophilin D (CYPD) ELISA Kit; PPID; CYP-40; CyP-D; PPIase D; Peptidylprolyl Isomerase D; Cyclophilin 40; 40 kDa peptidyl-prolyl cis-trans isomerase; Cyclophilin-related protein; Rotamase D; CYPD elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Cyclophilin D (CYPD).; No significant cross-reactivity or interference between Cyclophilin D (CYPD) and analogues was observed.
• Sequence Length: 370

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.125ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cyclophilin D (CYPD) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cyclophilin D (CYPD) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CYPD elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cyclophilin D (CYPD). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cyclophilin D (CYPD). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cyclophilin D (CYPD), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cyclophilin D (CYPD) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for CYPD elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 4826932
• NCBI GeneID: 5481
• NCBI Accession #: NP_005029.1
• NCBI GenBank Nucleotide #: NM_005038.2
• UniProt Accession #: Q08752; B2R9V2
• Molecular Weight: 40,764 Da
• NCBI Official Full Name: peptidyl-prolyl cis-trans isomerase D
• NCBI Official Synonym Full Names: peptidylprolyl isomerase D
• NCBI Official Symbol: PPID
• NCBI Official Synonym Symbols: CYPD; CYP-40
• NCBI Protein Information: peptidyl-prolyl cis-trans isomerase D
• UniProt Protein Name: Peptidyl-prolyl cis-trans isomerase D
• Protein Family: Cypemycin cysteine dehydrogenase
• UniProt Gene Name: PPID
• UniProt Synonym Gene Names: CYP40; CYPD; PPIase D; CYP-40

NCBI Description

The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein has been shown to possess PPIase activity and, similar to other family members, can bind to the immunosuppressant cyclosporin A. [provided by RefSeq, Jul 2008]

Uniprot Description

PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Proposed to act as a co-chaperone in HSP90 complexes such as in unligated steroid receptors heterocomplexes. Different co-chaperones seem to compete for association with HSP90 thus establishing distinct HSP90-co-chaperone-receptor complexes with the potential to exert tissue-specific receptor activity control. May have a preference for estrogen receptor complexes and is not found in glucocorticoid receptor complexes. May be involved in cytoplasmic dynein-dependent movement of the receptor from the cytoplasm to the nucleus. May regulate MYB by inhibiting its DNA-binding activity. Involved in regulation of AHR signaling by promoting the formation of the AHR:ARNT dimer; the function is independent of HSP90 but requires the chaperone activity. Involved in regulation of UV radiation-induced apoptosis. Promotes cell viability in anaplastic lymphoma kinase-positive anaplastic large-cell lymphoma (ALK+ ALCL) cell lines. May be involved in hepatitis C virus (HCV) replication and release.