Human Cyclin Dependent Kinase Inhibitor 3 (CDKN3) ELISA Kit | CDKN3 elisa kit

Human Cyclin Dependent Kinase Inhibitor 3 (CDKN3) ELISA Kit

Cat. Num. AAA2021946

• Gene Names: ICK6; inhibitor/interactor with cyclin-dependent kinase; K24G6.15; K24G6_15; KIP-RELATED PROTEIN 3; KRP3
• Reactivity: Human
• Synonyms: Cyclin Dependent Kinase Inhibitor 3 (CDKN3); Human Cyclin Dependent Kinase Inhibitor 3 (CDKN3) ELISA Kit; CDI1; CIP2; KAP1; CDK2-Associated Dual Specificity Phosphatase; Kinase Associated Phosphatase; Cyclin-dependent kinase interactor 1; Cyclin-dependent kinase-interacting 2; CDKN3 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Cyclin Dependent Kinase Inhibitor 3 (CDKN3).; No significant cross-reactivity or interference between Cyclin Dependent Kinase Inhibitor 3 (CDKN3) and analogues was observed.
• Sequence Length: 222

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.053ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cyclin Dependent Kinase Inhibitor 3 (CDKN3) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cyclin Dependent Kinase Inhibitor 3 (CDKN3) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CDKN3 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cyclin Dependent Kinase Inhibitor 3 (CDKN3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cyclin Dependent Kinase Inhibitor 3 (CDKN3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cyclin Dependent Kinase Inhibitor 3 (CDKN3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cyclin Dependent Kinase Inhibitor 3 (CDKN3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for CDKN3 elisa kit

Signal transduction; Enzyme & Kinase; Tumor immunity

NCBI and Uniprot Product Information

• NCBI GI #: 334188269
• NCBI GeneID: 834940
• NCBI Accession #: NP_001190495.1
• NCBI GenBank Nucleotide #: NM_001203566.1
• UniProt Accession #: Q9FKB5
• Molecular Weight: 24,925 Da
• NCBI Official Full Name: cyclin-dependent kinase inhibitor 3
• NCBI Official Symbol: ICK6
• NCBI Official Synonym Symbols: inhibitor/interactor with cyclin-dependent kinase; K24G6.15; K24G6_15; KIP-RELATED PROTEIN 3; KRP3
• NCBI Protein Information: cyclin-dependent kinase inhibitor 3
• UniProt Protein Name: Cyclin-dependent kinase inhibitor 3
• Protein Family: Cyclin-dependent kinase inhibitor
• UniProt Gene Name: KRP3
• UniProt Synonym Gene Names: ICK6
• UniProt Entry Name: KRP3_ARATH

Uniprot Description

Binds and inhibits CYCD2-1/CDKA-1 complex kinase activity. May target specifically CDKA-1.