Human Cyclin-Dependent Kinase 2 ELISA Kit | CDK2 elisa kit

Human Cyclin-Dependent Kinase 2 ELISA Kit

Cat. Num. AAA3803434

• Gene Names: CDK2; CDKN2; p33(CDK2)
• Reactivity: Human
• Synonyms: Cyclin-Dependent Kinase 2; Human Cyclin-Dependent Kinase 2 ELISA Kit; CDK2 elisa kit

• Reactivity: Human
• Sequence Length: 298

• Samples: Serum, plasma, cell culture supernate and other biological fluids
• Assay Type: Sandwich
• Detection Range: 4 mU/mL-64 mU/mL
• Sensitivity: 0.1 mU/mL

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CDK2 elisa kit

Intended Use: The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration ofCYCLIN-DEPENDENT KINASE 2in the sample, This CYCLIN-DEPENDENT KINASE 2ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versusCYCLIN-DEPENDENT KINASE 2concentration. The concentration ofCYCLIN-DEPENDENT KINASE 2in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 16936528
• NCBI GeneID: 1017
• NCBI Accession #: NP_001789
• NCBI GenBank Nucleotide #: NP_001789.2
• UniProt Accession #: P24941
• NCBI Official Full Name: cyclin-dependent kinase 2 isoform 1
• NCBI Official Synonym Full Names: cyclin dependent kinase 2
• NCBI Official Symbol: CDK2
• NCBI Official Synonym Symbols: CDKN2; p33(CDK2)
• NCBI Protein Information: cyclin-dependent kinase 2
• UniProt Protein Name: Cyclin-dependent kinase 2
• Protein Family: Cyclin-dependent kinase
• UniProt Gene Name: CDK2
• UniProt Synonym Gene Names: CDKN2
• UniProt Entry Name: CDK2_HUMAN

NCBI Description

This gene encodes a member of a family of serine/threonine protein kinases that participate in cell cycle regulation. The encoded protein is the catalytic subunit of the cyclin-dependent protein kinase complex, which regulates progression through the cell cycle. Activity of this protein is especially critical during the G1 to S phase transition. This protein associates with and regulated by other subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A), and p27Kip1 (CDKN1B). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014]

Uniprot Description

CDK2: a protein kinase of the CDK family. An important component of the cell cycle machinery. Activity of CDK2 is maximal during S phase and G2. Cdk2/cyclin E kinase activity is important for the G1 to S transition and phosphorylates the Rb protein. In S-phase, active cdk2/cyclin A complexes predominate and phosphorylate E2F, and the active cdk2 complex persists in the nucleus through G2. Part of the Rb pathway disregulated in most tumors. Target of several candidate cancer drugs. However, inhibition does not always prevent cancer cell growth, possibly due to CDK redundancy. Inhibitors: BMS-265246, BMS-265246-01, R-roscovitine (CYC200, CYC202), SU9516, R547, L868276

Protein type: Protein kinase, CMGC; EC 2.7.11.22; Protein kinase, Ser/Thr (non-receptor); Cell cycle regulation; Kinase, protein; CMGC group; CDK family; CDK1 subfamily; CDK/CDK1 subfamily

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: nucleoplasm; Y chromosome; Cajal body; centrosome; chromosome, telomeric region; transcription factor complex; X chromosome; cytoplasm; cyclin-dependent protein kinase holoenzyme complex; condensed chromosome; nucleus; cytosol; endosome

Molecular Function: cyclin binding; protein binding; cyclin-dependent protein kinase activity; metal ion binding; histone kinase activity; ATP binding

Biological Process: G1 DNA damage checkpoint; mitosis; meiosis; histone phosphorylation; positive regulation of transcription, DNA-dependent; DNA repair; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; peptidyl-serine phosphorylation; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; cell division; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; Ras protein signal transduction; positive regulation of cell proliferation; mitotic cell cycle; blood coagulation; DNA replication; G2/M transition of mitotic cell cycle; centrosome duplication; potassium ion transport; positive regulation of DNA replication initiation; G1/S transition of mitotic cell cycle