Typical Testing Data/Standard Curve (for reference only)
Human CTP synthase 1 (CTPS) ELISA Kit | CTPS elisa kit
Human CTP synthase 1 (CTPS) ELISA Kit
Gene Names
Ctps; Ctps1
Reactivity
Human
Synonyms
CTP synthase 1 (CTPS); Human CTP synthase 1 (CTPS) ELISA Kit; CTPS elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of CTPS1. No significant cross-reactivity or interference between CTPS1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CTPS1 and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
1.0 ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for CTPS elisa kit
Intended Uses: This CTPS1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CTPS1. This ELISA kit for research use only!
Principle of the Assay: CTPS1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CTPS1 antibody and an CTPS1-HRP conjugate. The assay sample and buffer are incubated together with CTPS1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CTPS1 concentration since CTPS1 from samples and CTPS1-HRP conjugate compete for the anti-CTPS1 antibody binding site. Since the number of sites is limited, as more sites are occupied by CTPS1 from the sample, fewer sites are left to bind CTPS1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CTPS1 concentration in each sample is interpolated from this standard curve.
Principle of the Assay: CTPS1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CTPS1 antibody and an CTPS1-HRP conjugate. The assay sample and buffer are incubated together with CTPS1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CTPS1 concentration since CTPS1 from samples and CTPS1-HRP conjugate compete for the anti-CTPS1 antibody binding site. Since the number of sites is limited, as more sites are occupied by CTPS1 from the sample, fewer sites are left to bind CTPS1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CTPS1 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
66,682 Da
NCBI Official Full Name
CTP synthase 1
NCBI Official Synonym Full Names
cytidine 5'-triphosphate synthase
NCBI Official Symbol
Ctps
NCBI Official Synonym Symbols
Ctps1
NCBI Protein Information
CTP synthase 1; CTP synthetase 1; UTP--ammonia ligase 1
UniProt Protein Name
CTP synthase 1
UniProt Gene Name
Ctps1
UniProt Entry Name
PYRG1_MOUSE
Uniprot Description
CTPS: the enzyme cytidine-5-prime-triphosphate synthetase converts UTP to CTP. Important in the biosynthesis of phospholipids and nucleic acids, and plays a key role in cell growth, development, and tumorigenesis. The region to which the CTPS gene has been mapped is the location of breakpoints involved in several tumor types.
Protein type: Motility/polarity/chemotaxis; Nucleotide Metabolism - pyrimidine; Ligase; EC 6.3.4.2
Cellular Component: membrane
Molecular Function: nucleotide binding; CTP synthase activity; ATP binding; ligase activity
Biological Process: T cell proliferation; B cell proliferation; CTP biosynthetic process; immune system process; glutamine metabolic process; pyrimidine nucleotide biosynthetic process
Research Articles on CTPS
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Product Notes
The Human CTPS ctps1 (Catalog #AAA7248343) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7248343 ELISA Kit recognizes Human CTPS. It is sometimes possible for the material contained within the vial of "CTP synthase 1 (CTPS), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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