Typical Testing Data/Standard Curve (for reference only)
(Human CRISP3 PicoKine ELISA Kit standard curve )
Human CRISP3 ELISA Kit | CRISP3 elisa kit
Human CRISP3 PicoKine ELISA Kit
Gene Names
CRISP3; Aeg2; CRS3; SGP28; CRISP-3; dJ442L6.3
Reactivity
Human
Synonyms
CRISP3; Human CRISP3 PicoKine ELISA Kit; Cysteine-rich secretory protein 3; cysteine-rich secretory protein 3; Aeg 2; Aeg2; CRIS3_HUMAN; CRISP 3; CRISP-3; Crisp3; CRS 3; CRS3; Cysteine rich secretory protein 3; dJ442L6.3; MGC126588; OTTHUMP00000016590; OTTHUMP00000039911; SGP 28; SGP28; SGP28 protein; Specific granule protein (28 kDa); Specific granule protein of 28 kDa; CRISP3 elisa kit
Reactivity
Human
Specificity
Natural and recombinant human CRISP3
Sequence Length
245
Samples
Cell culture supernates, serum, plasma (heparin, EDTA), saliva, urine and tissue homogenates
Assay Type
Sandwich
Detection Range
1.56ng/ml-100ng/ml
Sensitivity
< 50pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Typical Testing Data/Standard Curve (for reference only)
(Human CRISP3 PicoKine ELISA Kit standard curve )
Typical Testing Data/Standard Curve (for reference only)
(Human CRISP3 PicoKine ELISA Kit standard curve )
Related Product Information for CRISP3 elisa kit
Principle of the assay: human CRISP3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CRISP3 has been precoated onto 96-well plates. Standards(NSO, N21-Y245) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CRISP3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CRISP3 amount of sample captured in plate.
Background: Cysteine-rich secretory protein 3 is a cysteine-rich secretory protein that in humans is encoded by the CRISP3 gene. CRISP3 expression is found in the secretory epithelium throughout the male genital tract, with particularly high expression in the cauda epididymis and ampulla vas deferens. It is a potential biomarker for prostate cancer. CRISP3 gene can be defined as an early-transcribed gene with a putative role in the pathophysiology of sjogren's syndrome. CRISP3 is present in exocrine secretions and in secretory granules of neutrophilic granulocytes and is believed to play a role in innate immunity.
Background: Cysteine-rich secretory protein 3 is a cysteine-rich secretory protein that in humans is encoded by the CRISP3 gene. CRISP3 expression is found in the secretory epithelium throughout the male genital tract, with particularly high expression in the cauda epididymis and ampulla vas deferens. It is a potential biomarker for prostate cancer. CRISP3 gene can be defined as an early-transcribed gene with a putative role in the pathophysiology of sjogren's syndrome. CRISP3 is present in exocrine secretions and in secretory granules of neutrophilic granulocytes and is believed to play a role in innate immunity.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
30,116 Da
NCBI Official Full Name
Cysteine-rich secretory protein 3
NCBI Official Synonym Full Names
cysteine-rich secretory protein 3
NCBI Official Symbol
CRISP3
NCBI Official Synonym Symbols
Aeg2; CRS3; SGP28; CRISP-3; dJ442L6.3
NCBI Protein Information
cysteine-rich secretory protein 3; cysteine-rich secretory protein-3; specific granule protein (28 kDa); specific granule protein of 28 kDa
UniProt Protein Name
Cysteine-rich secretory protein 3
Protein Family
UniProt Gene Name
CRISP3
UniProt Synonym Gene Names
CRISP-3; SGP28
UniProt Entry Name
CRIS3_HUMAN
Uniprot Description
CRISP3: Belongs to the CRISP family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 6p12.3
Cellular Component: extracellular space; proteinaceous extracellular matrix; specific granule; extracellular region
Biological Process: innate immune response; defense response
Research Articles on CRISP3
Similar Products
Product Notes
The Human CRISP3 crisp3 (Catalog #AAA177209) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA177209 ELISA Kit recognizes Human CRISP3. It is sometimes possible for the material contained within the vial of "CRISP3, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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