Human Corticotropin Like Intermediate Lobe Peptide (CLIP) ELISA Kit | CLIP elisa kit

Human Corticotropin Like Intermediate Lobe Peptide (CLIP) ELISA Kit

Cat. Num. AAA2021001

• Reactivity: Human
• Synonyms: Corticotropin Like Intermediate Lobe Peptide (CLIP); Human Corticotropin Like Intermediate Lobe Peptide (CLIP) ELISA Kit; CLIP elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Corticotropin Like Intermediate Lobe Peptide (CLIP).; No significant cross-reactivity or interference between Corticotropin Like Intermediate Lobe Peptide (CLIP) and analogues was observed.

• Assay Type: Competitive Inhibition
• Samples: Serum, Plasma and Other Biological Fluids
• Detection Range: 12.35-1000pg/mL
• Sensitivity: < 4.89pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Corticotropin Like Intermediate Lobe Peptide (CLIP) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Corticotropin Like Intermediate Lobe Peptide (CLIP) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CLIP elisa kit

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Corticotropin Like Intermediate Lobe Peptide (CLIP) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Corticotropin Like Intermediate Lobe Peptide (CLIP) and unlabeled Corticotropin Like Intermediate Lobe Peptide (CLIP) (Standards or samples) with the pre-coated antibody specific to Corticotropin Like Intermediate Lobe Peptide (CLIP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Corticotropin Like Intermediate Lobe Peptide (CLIP) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Corticotropin Like Intermediate Lobe Peptide (CLIP) in the sample.