Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Cornifin-B (SPRR1B) Standard Curve.)
Human Cornifin-B (SPRR1B) ELISA Kit | SPRR1B elisa kit
Human Cornifin-B (SPRR1B) ELISA Kit
Gene Names
SPRR1B; SPRR1; GADD33; SPR-IB; CORNIFIN
Reactivity
Human
Synonyms
Cornifin-B (SPRR1B); Human Cornifin-B (SPRR1B) ELISA Kit; SPRR1B; CORNIFIN; GADD33; MGC61901; SPRR1; cornifin B; small proline-rich protein 1B; SPRR1B elisa kit
Reactivity
Human
Specificity
Human Cornifin-B (SPRR1B) ELISA Kit has high sensitivity and excellent specificity for detection of Human SPRR1B. No significant cross-reactivity or interference between Human SPRR1B and analogues was observed.
Sequence Length
89
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Cornifin-B (SPRR1B) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Cornifin-B (SPRR1B) Standard Curve.)
Related Product Information for SPRR1B elisa kit
Description: This Human Cornifin-B (SPRR1B) ELISA Kit employs a two-site sandwich ELISA to quantitate SPRR1B in samples. An antibody specific for SPRR1B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SPRR1B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPRR1B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPRR1B bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: All SPRR genes are closely linked within a 300-kb DNA segment on 1q21-q22.Marvin et al. (1992) characterized a cDNA clone corresponding to mRNA abundantly expressed in differentiating squamous cells originally isolated from a rabbit squamous cell library. Small Proline Rich Protein 1B is a 14-kD protein that appears to function as a component of the crosslinked envelope in squamous differentiating cells. Called cornifin, the protein has a high content of proline (31%), glutamine (20%), and cysteine (11%) and contains 13 repeats of an octapeptide (consensus sequence, EPCQPKVP) at its C terminus. In situ hybridization analysis showed that the mRNA is restricted to the suprabasal layers of the epidermis. Crosslinking of the protein appears to be mediated by transglutaminase type I.
Background: All SPRR genes are closely linked within a 300-kb DNA segment on 1q21-q22.Marvin et al. (1992) characterized a cDNA clone corresponding to mRNA abundantly expressed in differentiating squamous cells originally isolated from a rabbit squamous cell library. Small Proline Rich Protein 1B is a 14-kD protein that appears to function as a component of the crosslinked envelope in squamous differentiating cells. Called cornifin, the protein has a high content of proline (31%), glutamine (20%), and cysteine (11%) and contains 13 repeats of an octapeptide (consensus sequence, EPCQPKVP) at its C terminus. In situ hybridization analysis showed that the mRNA is restricted to the suprabasal layers of the epidermis. Crosslinking of the protein appears to be mediated by transglutaminase type I.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
cornifin-B
NCBI Official Synonym Full Names
small proline rich protein 1B
NCBI Official Symbol
SPRR1B
NCBI Official Synonym Symbols
SPRR1; GADD33; SPR-IB; CORNIFIN
NCBI Protein Information
cornifin-B
UniProt Protein Name
Cornifin-B
Protein Family
UniProt Gene Name
SPRR1B
UniProt Synonym Gene Names
SPR-IB
UniProt Entry Name
SPR1B_HUMAN
NCBI Description
The protein encoded by this gene is an envelope protein of keratinocytes. The encoded protein is crosslinked to membrane proteins by transglutaminase, forming an insoluble layer under the plasma membrane. This protein is proline-rich and contains several tandem amino acid repeats. [provided by RefSeq, Nov 2015]
Uniprot Description
SPRR1B: Cross-linked envelope protein of keratinocytes. It is a keratinocyte protein that first appears in the cell cytosol, but ultimately becomes cross-linked to membrane proteins by transglutaminase. All that results in the formation of an insoluble envelope beneath the plasma membrane. Can function as both amine donor and acceptor in transglutaminase-mediated cross- linkage. Belongs to the cornifin (SPRR) family.
Protein type: Adaptor/scaffold
Chromosomal Location of Human Ortholog: 1q21-q22
Cellular Component: cytoplasm; cornified envelope
Molecular Function: protein binding, bridging; structural molecule activity
Biological Process: keratinocyte differentiation; epidermis development; keratinization; peptide cross-linking
Research Articles on SPRR1B
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Product Notes
The Human SPRR1B sprr1b (Catalog #AAA9714013) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714013 ELISA Kit recognizes Human SPRR1B. It is sometimes possible for the material contained within the vial of "Cornifin-B (SPRR1B), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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