Human Corin (CRN) ELISA Kit | CRN elisa kit

Human Corin (CRN) ELISA Kit

Cat. Num. AAA2023035

• Gene Names: CORIN; CRN; ATC2; Lrp4; PEE5; TMPRSS10
• Reactivity: Human
• Synonyms: Corin (CRN); Human Corin (CRN) ELISA Kit; ATC2; CRN; Lrp4; TMPRSS10; Atrial Natriuretic Peptide-Converting Enzyme; Serine Protease; Heart-specific serine proteinase ATC2; Pro-ANP-converting enzyme; CRN elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Corin (CRN).; No significant cross-reactivity or interference between Corin (CRN) and analogues was observed.
• Sequence Length: 1,013

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.059ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Corin (CRN) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Corin (CRN) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CRN elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Corin (CRN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Corin (CRN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Corin (CRN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Corin (CRN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for CRN elisa kit

Enzyme & Kinase

NCBI and Uniprot Product Information

• NCBI GI #: 4884872
• NCBI GeneID: 10699
• UniProt Accession #: Q9Y5Q5; Q2TBD2; Q4W5E5; Q4W5G6; Q9UHY2; B0ZBE3
• Molecular Weight: 113,297 Da
• NCBI Official Full Name: corin
• NCBI Official Synonym Full Names: corin, serine peptidase
• NCBI Official Symbol: CORIN
• NCBI Official Synonym Symbols: CRN; ATC2; Lrp4; PEE5; TMPRSS10
• NCBI Protein Information: atrial natriuretic peptide-converting enzyme
• UniProt Protein Name: Atrial natriuretic peptide-converting enzyme
• Protein Family: Protein crooked neck
• UniProt Gene Name: CORIN
• UniProt Synonym Gene Names: CRN; TMPRSS10
• UniProt Entry Name: CORIN_HUMAN

NCBI Description

This gene encodes a member of the type II transmembrane serine protease class of the trypsin superfamily. Members of this family are composed of multiple structurally distinct domains. The encoded protein converts pro-atrial natriuretic peptide to biologically active atrial natriuretic peptide, a cardiac hormone that regulates blood volume and pressure. This protein may also function as a pro-brain-type natriuretic peptide convertase. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2013]

Uniprot Description

CORIN: Serine-type endopeptidase involved in atrial natriuretic peptide hormone (NPPA) processing. Converts through proteolytic cleavage the non-functional propeptide NPPA into the active hormone, thereby regulating blood pressure in heart and promoting natriuresis, diuresis and vasodilation. Proteolytic cleavage of pro-NPPA also plays a role in female pregnancy by promoting trophoblast invasion and spiral artery remodeling in uterus. Also acts as a regulator of sodium reabsorption in kidney. May also process pro-NPPB the B-type natriuretic peptide. Defects in CORIN are the cause of pre-eclampsia/eclampsia 5 (PEE5); also known as gestational proteinuric hypertension. Preeclampsia is a pregnancy-associated disease with maternal symptoms but placental origin. Unlike most other human disorders, it impacts 2 individuals, the mother and her child, both of whom can be severely affected. Belongs to the peptidase S1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.21.-; Membrane protein, integral; Protease; Cell surface

Chromosomal Location of Human Ortholog: 4p12

Cellular Component: cell surface; integral to plasma membrane; integral to membrane; plasma membrane; extracellular region

Molecular Function: serine-type endopeptidase activity

Biological Process: regulation of blood pressure; peptide hormone processing; female pregnancy; proteolysis; regulation of systemic arterial blood pressure by atrial natriuretic peptide

Disease: Preeclampsia/eclampsia 5