Human Complement Component 4a (C4a) ELISA Kit | C4a elisa kit

Human Complement Component 4a (C4a) ELISA Kit

Cat. Num. AAA456028

• Gene Names: C4B; CH; C4F; CO4; C4B1; C4B2; C4B3; C4B5; C4BD; C4B12; C4B_2; CPAMD3
• Reactivity: Human
• Synonyms: Complement Component 4a (C4a); Human Complement Component 4a (C4a) ELISA Kit; C4; C4S; CO4; CPAMD2; RG; C4A; C4F; CH; CPAMD3; C4(Rodgers Blood Group); Acidic C4; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 2; C4a elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of C4a. No significant cross-reactivity or interference between C4a and analogues was observed.
• Sequence Length: 1,698

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.058ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level C4a were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level C4a were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for C4a elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of C4a in human serum, plasma and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to C4a. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to C4a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain C4a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of C4a in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 178557739
• NCBI GeneID: 721
• NCBI Accession #: NP_001002029.3
• NCBI GenBank Nucleotide #: NM_001002029.3
• UniProt Accession #: P0C0L4; P01028; P78445; Q13160; Q13906; A6H8M8; A6NHJ5; A7E2V2; B0QZR6; B0V2C8; B2RUT6; B7ZVZ6; P0C0L5
• Molecular Weight: 187,704 Da
• NCBI Official Full Name: complement C4-B preproprotein
• NCBI Official Synonym Full Names: complement C4B (Chido blood group)
• NCBI Official Symbol: C4B
• NCBI Official Synonym Symbols: CH; C4F; CO4; C4B1; C4B2; C4B3; C4B5; C4BD; C4B12; C4B_2; CPAMD3
• NCBI Protein Information: complement C4-B
• UniProt Protein Name: Complement C4-A
• Protein Family: Complement C4
• UniProt Gene Name: C4A
• UniProt Synonym Gene Names: CO4; CPAMD2

NCBI Description

This gene encodes the basic form of complement factor 4, part of the classical activation pathway. The protein is expressed as a single chain precursor which is proteolytically cleaved into a trimer of alpha, beta, and gamma chains prior to secretion. The trimer provides a surface for interaction between the antigen-antibody complex and other complement components. The alpha chain may be cleaved to release C4 anaphylatoxin, a mediator of local inflammation. Deficiency of this protein is associated with systemic lupus erythematosus. This gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. Varying haplotypes of this gene cluster exist, such that individuals may have 1, 2, or 3 copies of this gene. In addition, this gene exists as a long form and a short form due to the presence or absence of a 6.4 kb endogenous HERV-K retrovirus in intron 9. [provided by RefSeq, Jul 2008]

Uniprot Description

Non-enzymatic component of C3 and C5 convertases and thus essential for the propagation of the classical complement pathway. Covalently binds to immunoglobulins and immune complexes and enhances the solubilization of immune aggregates and the clearance of IC through CR1 on erythrocytes. C4A isotype is responsible for effective binding to form amide bonds with immune aggregates or protein antigens, while C4B isotype catalyzes the transacylation of the thioester carbonyl group to form ester bonds with carbohydrate antigens.