Human Collagen Type III Alpha 1 (COL3A1) ELISA Kit | COL3A1 elisa kit

Human Collagen Type III Alpha 1 (COL3A1) ELISA Kit

Cat. Num. AAA9716169

• Gene Names: COL3A1; EDS4A; EDSVASC; PMGEDSV
• Reactivity: Human
• Synonyms: Collagen Type III Alpha 1 (COL3A1); Human Collagen Type III Alpha 1 (COL3A1) ELISA Kit; COL3A1 elisa kit

• Reactivity: Human
• Sequence Length: 1466

• Assay Type: Sandwich ELISA
• Samples: Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
• Detection Range: 37.5 pg/mL-600 pg/mL
• Detection Method: Colorimetric
• Limit of Detection: 1.0 pg/mL
• Usage Notes: * Do not mix components from different kit lots or use reagents beyond the kit expiration date.; * Allow all reagents to warm to room temperature for at least 30 minutes before opening.; * Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.; * Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.; * Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.; * It is recommended that all samples and standards be assayed in duplicate or triplicate.
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for COL3A1 elisa kit

Human Collagen Type III Alpha 1 (COL3A1) ELISA Kit employs a two-site sandwich ELISA to quantitate COL3A1 in samples. An antibody specific for COL3A1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any COL3A1 present is bound by the immobilized antibody. After removing any unbound substances, HRP-Conjugate Human COL3A1 detection antibody is added to the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proportion to the amount of COL3A1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 494319369
• NCBI GeneID: 1281
• NCBI Official Full Name: collagen type III alpha 1
• NCBI Official Synonym Full Names: collagen type III alpha 1 chain
• NCBI Official Symbol: COL3A1
• NCBI Official Synonym Symbols: EDS4A; EDSVASC; PMGEDSV
• NCBI Protein Information: collagen alpha-1(III) chain
• Protein Family: Collagen

NCBI Description

This gene encodes the pro-alpha1 chains of type III collagen, a fibrillar collagen that is found in extensible connective tissues such as skin, lung, uterus, intestine and the vascular system, frequently in association with type I collagen. Mutations in this gene are associated with Ehlers-Danlos syndrome types IV, and with aortic and arterial aneurysms. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. [provided by R. Dalgleish, Feb 2008]