Human Cold Shock Domain Containing Protein E1, RNA Binding (CSDE1) ELISA Kit | CSDE1 elisa kit

Human Cold Shock Domain Containing Protein E1, RNA Binding (CSDE1) ELISA Kit

Cat. Num. AAA2023465

• Gene Names: CSDE1; UNR; D1S155E
• Reactivity: Human
• Synonyms: Cold Shock Domain Containing Protein E1; RNA Binding (CSDE1); Human Cold Shock Domain Containing Protein E1; RNA Binding (CSDE1) ELISA Kit; UNR; NRU; Upstream of NRAS; N-ras upstream gene protein; CSDE1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Cold Shock Domain Containing Protein E1, RNA Binding (CSDE1).; No significant cross-reactivity or interference between Cold Shock Domain Containing Protein E1, RNA Binding (CSDE1) and analogues was observed.
• Sequence Length: 844

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.122ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cold Shock Domain Containing Protein E1, RNA Binding (CSDE1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cold Shock Domain Containing Protein E1, RNA Binding (CSDE1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CSDE1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cold Shock Domain Containing Protein E1, RNA Binding (CSDE1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cold Shock Domain Containing Protein E1, RNA Binding (CSDE1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cold Shock Domain Containing Protein E1, RNA Binding (CSDE1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cold Shock Domain Containing Protein E1, RNA Binding (CSDE1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for CSDE1 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 194473720
• NCBI GeneID: 7812
• NCBI Accession #: NP_001123995.1
• NCBI GenBank Nucleotide #: NM_001130523.2
• UniProt Accession #: O75534; O94961; Q5TF04; Q5TF05; Q68DF1; Q68DI9; Q9Y2S4; A8K281; E9PGZ0; G5E9Q2
• Molecular Weight: 93,742 Da
• NCBI Official Full Name: cold shock domain-containing protein E1 isoform 3
• NCBI Official Synonym Full Names: cold shock domain containing E1, RNA-binding
• NCBI Official Symbol: CSDE1
• NCBI Official Synonym Symbols: UNR; D1S155E
• NCBI Protein Information: cold shock domain-containing protein E1
• UniProt Protein Name: Cold shock domain-containing protein E1
• Protein Family: Cold shock domain-containing protein
• UniProt Gene Name: CSDE1
• UniProt Synonym Gene Names: D1S155E; KIAA0885; NRU; UNR
• UniProt Entry Name: CSDE1_HUMAN

Uniprot Description

CSDE1: RNA-binding protein. Required for internal initiation of translation of human rhinovirus RNA. May be involved in translationally coupled mRNA turnover. Implicated with other RNA- binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding- region determinant of instability (mCRD) domain. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding; Mitochondrial; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 1p22

Cellular Component: Golgi apparatus; mitochondrial inner membrane; cytoplasm; plasma membrane

Molecular Function: DNA binding

Biological Process: regulation of transcription, DNA-dependent; male gonad development