Typical Testing Data/Standard Curve (for reference only)
Human COL2alpha1 ELISA Kit | COL2alpha1 elisa kit
Human COL2alpha1 (Collagen Type II Alpha 1) ELISA Kit
Gene Names
Col2a1; CG2A1A; COLLII
Reactivity
Human
Synonyms
COL2alpha1; Human COL2alpha1 (Collagen Type II Alpha 1) ELISA Kit; COL2alpha1 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Human COL2alpha1. No significant cross-reactivity or interference between Human COL2alpha1 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human COL2alpha1 and all the analogues, therefore, cross reaction may still exist.
Sequence Length
1419
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
0.625-40ng/mL
Sensitivity
Min: 0.375ng/mL; Max: 40ng/mL
Preparation and Storage
Store at 4 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for COL2alpha1 elisa kit
Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of COL2alpha1 in human serum, plasma and other biological fluids
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human COL2alpha1. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human COL2alpha1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human COL2alpha1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human COL2alpha1. You can calculate the concentration of Human COL2alpha1 in the samples by comparing the OD of the samples to the standard curve.
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human COL2alpha1. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human COL2alpha1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human COL2alpha1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human COL2alpha1. You can calculate the concentration of Human COL2alpha1 in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
134,570 Da
NCBI Official Full Name
collagen alpha-1(II) chain
NCBI Official Synonym Full Names
collagen, type II, alpha 1
NCBI Official Symbol
Col2a1
NCBI Official Synonym Symbols
CG2A1A; COLLII
NCBI Protein Information
collagen alpha-1(II) chain; collagen alpha-1(II) chain; Procollagen II alpha 1; alpha-1 type II collagen; collagen type II (Col2A1) gene, enhancer region; procollagen, type II, alpha 1
UniProt Protein Name
Collagen alpha-1(II) chain
UniProt Gene Name
Col2a1
UniProt Entry Name
CO2A1_RAT
NCBI Description
structural component of the cartilage extracellular matrix [RGD, Feb 2006]
Uniprot Description
Type II collagen is specific for cartilaginous tissues. It is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces.
Research Articles on COL2alpha1
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Product Notes
The Human COL2alpha1 col2a1 (Catalog #AAA2500568) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2500568 ELISA Kit recognizes Human COL2alpha1. It is sometimes possible for the material contained within the vial of "COL2alpha1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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