Human coagulation factor VII, FVII ELISA Kit | FVII elisa kit

Human coagulation factor VII, FVII ELISA Kit

Cat. Num. AAA164745

• Gene Names: F7; SPCA
• Reactivity: Human
• Synonyms: coagulation factor VII; FVII; Human coagulation factor VII; FVII ELISA Kit; FVII elisa kit

• Reactivity: Human
• Sequence Length: 21

• Samples: Serum, plasma, cell culture supernates, cell lysates, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 0.2ng/ml - 70ng/ml
• Sensitivity: 0.093ng/ml
• Intra-Assay: CV<8%
• Inter-Assay: CV<10%
• Preparation and Storage: Store at 2 to 8 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FVII elisa kit

Intended Uses: This sandwich kit is for the accurate quantitative detection of human Coagulation factor VII (also known as FVII) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human FVII antibody. FVII present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human FVII Antibody is added and binds to FVII in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated FVII antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human FVII. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

NCBI and Uniprot Product Information

• NCBI GI #: 12750767
• NCBI GeneID: 2155
• UniProt Accession #: P08709; Q14339; Q5JVF1; Q5JVF2; Q9UD52; Q9UD53; Q9UD54; B0YJC8
• Molecular Weight: 49,320 Da
• NCBI Official Full Name: coagulation factor VII, partial
• NCBI Official Synonym Full Names: coagulation factor VII
• NCBI Official Symbol: F7
• NCBI Official Synonym Symbols: SPCA
• NCBI Protein Information: coagulation factor VII
• UniProt Protein Name: Coagulation factor VII
• UniProt Gene Name: F7
• UniProt Synonym Gene Names: SPCA

NCBI Description

This gene encodes coagulation factor VII which is a vitamin K-dependent factor essential for hemostasis. This factor circulates in the blood in a zymogen form, and is converted to an active form by either factor IXa, factor Xa, factor XIIa, or thrombin by minor proteolysis. Upon activation of the factor VII, a heavy chain containing a catalytic domain and a light chain containing 2 EGF-like domains are generated, and two chains are held together by a disulfide bond. In the presence of factor III and calcium ions, the activated factor then further activates the coagulation cascade by converting factor IX to factor IXa and/or factor X to factor Xa. Defects in this gene can cause coagulopathy. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar proteolytic processing to generate mature polypeptides. [provided by RefSeq, Aug 2015]

Uniprot Description

Initiates the extrinsic pathway of blood coagulation. Serine protease that circulates in the blood in a zymogen form. Factor VII is converted to factor VIIa by factor Xa, factor XIIa, factor IXa, or thrombin by minor proteolysis. In the presence of tissue factor and calcium ions, factor VIIa then converts factor X to factor Xa by limited proteolysis. Factor VIIa will also convert factor IX to factor IXa in the presence of tissue factor and calcium.