Human Cluster Of Differentiation 5 (CD5) ELISA Kit | CD5 elisa kit

Human Cluster Of Differentiation 5 (CD5) ELISA Kit

Cat. Num. AAA2122020

• Gene Names: CD5; T1; LEU1
• Reactivity: Human
• Synonyms: Cluster Of Differentiation 5 (CD5); Human Cluster Of Differentiation 5 (CD5) ELISA Kit; T1; LEU1; Lymphocyte antigen T1/Leu-1; CD5 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Cluster Of Differentiation 5 (CD5).; No significant cross-reactivity or interference between Cluster Of Differentiation 5 (CD5) and analogues was observed.
• Sequence Length: 495

• Assay Type: Double-antibody Sandwich
• Sample: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and Other Biological Fluids
• Dectection Range: 0.78-50ng/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 0.27ng/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cluster Of Differentiation 5 (CD5) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cluster Of Differentiation 5 (CD5) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CD5 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cluster Of Differentiation 5 (CD5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cluster Of Differentiation 5 (CD5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cluster Of Differentiation 5 (CD5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cluster Of Differentiation 5 (CD5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for CD5 elisa kit

Signal Transduction; CD & Adhesion Molecule; Infection Immunity; Immune Molecule

NCBI and Uniprot Product Information

• NCBI GI #: 166197668
• NCBI GeneID: 921
• NCBI Accession #: NP_055022.2
• NCBI GenBank Nucleotide #: NP_055022.2
• UniProt Accession #: P06127
• NCBI Official Full Name: T-cell surface glycoprotein CD5 isoform 1
• NCBI Official Synonym Full Names: CD5 molecule
• NCBI Official Symbol: CD5
• NCBI Official Synonym Symbols: T1; LEU1
• NCBI Protein Information: T-cell surface glycoprotein CD5
• UniProt Protein Name: T-cell surface glycoprotein CD5
• Protein Family: T-cell surface glycoprotein
• UniProt Gene Name: CD5
• UniProt Synonym Gene Names: LEU1
• UniProt Entry Name: CD5_HUMAN

NCBI Description

This gene encodes a member of the scavenger receptor cysteine-rich (SRCR) superfamily. Members of this family are secreted or membrane-anchored proteins mainly found in cells associated with the immune system. This protein is a type-I transmembrane glycoprotein found on the surface of thymocytes, T lymphocytes and a subset of B lymphocytes. The encoded protein contains three SRCR domains and may act as a receptor to regulate T-cell proliferation. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2016]

Uniprot Description

CD5: a surface glycoprotein expressed on T cells and B-1 B cells. Rapidly recruited to the immunological synapse, lowering the response of the T cell antigen receptor. CD5 interacts with CD72/LYB-2. Promotes B-cell survival through stimulation of autocrine IL-10 production. Contains 3 SRCR domains.

Protein type: Cell surface; Membrane protein, integral

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: integral to plasma membrane; plasma membrane; external side of plasma membrane

Molecular Function: protein binding; transmembrane receptor activity; receptor activity; scavenger receptor activity

Biological Process: receptor-mediated endocytosis; cell proliferation; T cell costimulation; cell recognition