Human Cluster Of Differentiation 109 (CD109) ELISA Kit | CD109 elisa kit

Human Cluster Of Differentiation 109 (CD109) ELISA Kit

Cat. Num. AAA2024379

• Gene Names: CD109; p180; r150; CPAMD7
• Reactivity: Human
• Synonyms: Cluster Of Differentiation 109 (CD109); Human Cluster Of Differentiation 109 (CD109) ELISA Kit; CPAMD7; p180; r150; Gov platelet alloantigens; 150 kDa TGF-beta-1-binding protein; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 7; CD109 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Cluster Of Differentiation 109 (CD109).; No significant cross-reactivity or interference between Cluster Of Differentiation 109 (CD109) and analogues was observed.
• Sequence Length: 1,428

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.057ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cluster Of Differentiation 109 (CD109) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cluster Of Differentiation 109 (CD109) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CD109 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cluster Of Differentiation 109 (CD109). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cluster Of Differentiation 109 (CD109). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cluster Of Differentiation 109 (CD109), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cluster Of Differentiation 109 (CD109) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for CD109 elisa kit

CD & Adhesion molecule; Tumor immunity; Infection immunity; Hematology; Autoimmunity

NCBI and Uniprot Product Information

• NCBI GI #: 227430303
• NCBI GeneID: 135228
• NCBI Accession #: NP_001153060.1
• NCBI GenBank Nucleotide #: NM_001159588.2
• UniProt Accession #: Q6YHK3; Q0P6K7; Q5SYA8; Q5XUM7; Q5XUM9; Q6MZI7; Q8N3A7; Q8N915; Q8TDJ2; A5YKK4; B2R948; B3KW25
• Molecular Weight: 159,696 Da
• NCBI Official Full Name: CD109 antigen isoform 3 preproprotein
• NCBI Official Synonym Full Names: CD109 molecule
• NCBI Official Symbol: CD109
• NCBI Official Synonym Symbols: p180; r150; CPAMD7
• NCBI Protein Information: CD109 antigen
• UniProt Protein Name: CD109 antigen
• Protein Family: CD109 antigen
• UniProt Gene Name: CD109
• UniProt Synonym Gene Names: CPAMD7
• UniProt Entry Name: CD109_HUMAN

NCBI Description

This gene encodes a glycosyl phosphatidylinositol (GPI)-linked glycoprotein that localizes to the surface of platelets, activated T-cells, and endothelial cells. The protein binds to and negatively regulates signalling by transforming growth factor beta (TGF-beta). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2014]

Uniprot Description

CD109: Modulates negatively TGFB1 signaling in keratinocytes. Belongs to the protease inhibitor I39 (alpha-2- macroglobulin) family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 6q13

Cellular Component: extracellular space; cell surface; plasma membrane

Molecular Function: serine-type endopeptidase inhibitor activity; transforming growth factor beta binding

Biological Process: negative regulation of protein amino acid phosphorylation; hair follicle development; negative regulation of transforming growth factor beta receptor signaling pathway; regulation of keratinocyte differentiation