Human Chromosome associated kinesin KIF4A (KIF4A) ELISA Kit | KIF4A elisa kit

Human Chromosome associated kinesin KIF4A (KIF4A) ELISA Kit

Cat. Num. AAA7222157

• Gene Names: KIF4A; KIF4; KIF4G1; MRX100
• Reactivity: Human
• Synonyms: Chromosome associated kinesin KIF4A (KIF4A); Human Chromosome associated kinesin KIF4A (KIF4A) ELISA Kit; KIF4A elisa kit

• Reactivity: Human

• Samples: Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate
• Assay Type: Quantitative Competitive
• Sensitivity: 0.1 ng/mL.
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for KIF4A elisa kit

Intended Uses: This Ki-67 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Goat Ki-67. This ELISA kit for research use only!

Principle of the Assay: Ki-67 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-Ki-67 antibody and an Ki-67-HRP conjugate. The assay sample and buffer are incubated together with Ki-67-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Ki-67 concentration since Ki-67 from samples and Ki-67-HRP conjugate compete for the anti-Ki-67 antibody binding site. Since the number of sites is limited, as more sites are occupied by Ki-67 from the sample, fewer sites are left to bind Ki-67-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Ki-67 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for KIF4A elisa kit

Cell Biology

NCBI and Uniprot Product Information

• NCBI GI #: 116686122
• NCBI GeneID: 24137
• NCBI Accession #: NP_036442.3
• NCBI GenBank Nucleotide #: NM_012310.4
• Molecular Weight: 128,459 Da
• NCBI Official Full Name: chromosome-associated kinesin KIF4A
• NCBI Official Synonym Full Names: kinesin family member 4A
• NCBI Official Symbol: KIF4A
• NCBI Official Synonym Symbols: KIF4; KIF4G1; MRX100
• NCBI Protein Information: chromosome-associated kinesin KIF4A; chromokinesin-A
• UniProt Protein Name: Chromosome-associated kinesin KIF4A
• Protein Family: Chromosome-associated kinesin
• UniProt Gene Name: KIF4A
• UniProt Synonym Gene Names: KIF4
• UniProt Entry Name: KIF4A_HUMAN

NCBI Description

This gene encodes a member of the kinesin 4 subfamily of kinesin related proteins. The encoded protein is an ATP dependent microtubule-based motor protein that is involved in the intracellular transport of membranous organelles. This protein also associates with condensed chromosome arms and may be involved in maintaining chromosome integrity during mitosis. This protein may also be involved in the organization of the central spindle prior to cytokinesis. A pseudogene of this gene is found on chromosome X.[provided by RefSeq, Mar 2010]

Uniprot Description

KIF4A: Motor protein that translocates PRC1 to the plus ends of interdigitating spindle microtubules during the metaphase to anaphase transition, an essential step for the formation of an organized central spindle midzone and midbody and for successful cytokinesis. May play a role in mitotic chromosomal positioning and bipolar spindle stabilization. Belongs to the kinesin-like protein family. Chromokinesin subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motor; Microtubule-binding

Chromosomal Location of Human Ortholog: Xq13.1

Cellular Component: nucleoplasm; kinesin complex; nuclear matrix; membrane; spindle microtubule; cytoplasm; midbody; chromosome; cytosol

Molecular Function: protein binding; DNA binding; plus-end-directed microtubule motor activity; microtubule binding; ATPase activity; microtubule motor activity; ATP binding

Biological Process: axon guidance; cytokinesis after mitosis; spindle midzone assembly involved in mitosis; metabolic process; organelle organization and biogenesis; antigen processing and presentation of exogenous peptide antigen via MHC class II; anterograde axon cargo transport; blood coagulation; microtubule-based movement

Disease: Mental Retardation, X-linked 100