Human Cholinergic Receptor, Nicotinic, Alpha 1 (CHRNa1) ELISA Kit | CHRNa1 elisa kit

Human Cholinergic Receptor, Nicotinic, Alpha 1 (CHRNa1) ELISA Kit

Cat. Num. AAA453681

• Gene Names: CHRNA1; ACHRA; ACHRD; CHRNA; CMS1A; CMS1B; CMS2A; FCCMS; SCCMS
• Reactivity: Human
• Synonyms: Cholinergic Receptor; Nicotinic; Alpha 1 (CHRNa1); Human Cholinergic Receptor; Alpha 1 (CHRNa1) ELISA Kit; CHR-NA1 N-AChRA1 NAChRA1 ACHRA ACHRD CMS2A FCCMS SCCMS Acetylcholine receptor subunit alpha N-AChR-A1 Neuronal Acetylcholine Receptor Alpha 1 Muscle; CHRNa1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of CHRNa1. No significant cross-reactivity or interference between CHRNa1 and analogues was observed.
• Sequence Length: 457

• Samples: Tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.781-50ng/mL
• Sensitivity: 0.28ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CHRNa1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CHRNa1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CHRNa1 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CHRNa1 in human tissue homogenates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CHRNa1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to CHRNa1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CHRNa1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of CHRNa1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4557457
• NCBI GeneID: 1134
• NCBI Accession #: NP_000070.1
• NCBI GenBank Nucleotide #: NM_000079.3
• UniProt Accession #: P02708; B4DRV6; D3DPE8
• Molecular Weight: 51,839 Da
• NCBI Official Full Name: acetylcholine receptor subunit alpha isoform b
• NCBI Official Synonym Full Names: cholinergic receptor nicotinic alpha 1 subunit
• NCBI Official Symbol: CHRNA1
• NCBI Official Synonym Symbols: ACHRA; ACHRD; CHRNA; CMS1A; CMS1B; CMS2A; FCCMS; SCCMS
• NCBI Protein Information: acetylcholine receptor subunit alpha
• UniProt Protein Name: Acetylcholine receptor subunit alpha
• Protein Family: Acetylcholine receptor
• UniProt Gene Name: CHRNA1
• UniProt Synonym Gene Names: ACHRA; CHNRA

NCBI Description

The muscle acetylcholine receptor consiststs of 5 subunits of 4 different types: 2 alpha subunits and 1 each of the beta, gamma, and delta subunits. This gene encodes an alpha subunit that plays a role in acetlycholine binding/channel gating. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Nov 2012]

Uniprot Description

After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.