Typical Testing Data/Standard Curve (for reference only)
Human Choline kinase Alpha (CHKA) ELISA Kit | CHKA elisa kit
Human Choline kinase Alpha (CHKA) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: CHKA ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-CHKA antibody and an CHKA-HRP conjugate. The assay sample and buffer are incubated together with CHKA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CHKA concentration since CHKA from samples and CHKA-HRP conjugate compete for the anti-CHKA antibody binding site. Since the number of sites is limited, as more sites are occupied by CHKA from the sample, fewer sites are left to bind CHKA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CHKA concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
The major pathway for the biosynthesis of phosphatidylcholine occurs via the CDP-choline pathway. The protein encoded by this gene is the initial enzyme in the sequence and may play a regulatory role. The encoded protein also catalyzes the phosphorylation of ethanolamine. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
CHKA: Has a key role in phospholipid biosynthesis and may contribute to tumor cell growth. Catalyzes the first step in phosphatidylcholine biosynthesis. Contributes to phosphatidylethanolamine biosynthesis. Phosphorylates choline and ethanolamine. Has higher activity with choline. Belongs to the choline/ethanolamine kinase family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Lipid Metabolism - glycerophospholipid; Kinase, other; EC 2.7.1.32; EC 2.7.1.82
Chromosomal Location of Human Ortholog: 11q13.2
Cellular Component: cytosol
Molecular Function: choline binding; signal transducer activity; protein homodimerization activity; cholinesterase activity; drug binding; choline kinase activity; ethanolamine kinase activity; ATP binding
Biological Process: choline metabolic process; CDP-choline pathway; phospholipid metabolic process; glycerophospholipid biosynthetic process; phosphatidylethanolamine biosynthetic process; phosphatidylcholine biosynthetic process; lipid metabolic process; lipid transport; signal transduction; phosphorylation
Research Articles on CHKA
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Product Notes
The Human CHKA chka (Catalog #AAA7232272) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7232272 ELISA Kit recognizes Human CHKA. It is sometimes possible for the material contained within the vial of "Choline kinase Alpha (CHKA), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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