Human Chloride intracellular channel protein 2 (CLIC2) ELISA Kit | CLIC2 elisa kit

Human Chloride intracellular channel protein 2 (CLIC2) ELISA Kit

Cat. Num. AAA7203397

• Gene Names: CLIC2; CLIC2b; MRXS32; XAP121
• Reactivity: Human
• Synonyms: Chloride intracellular channel protein 2 (CLIC2); Human Chloride intracellular channel protein 2 (CLIC2) ELISA Kit; CLIC2 elisa kit

• Reactivity: Human

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 250-5000pg/mL
• Sensitivity: 1.0pg/mL
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Standard Curve/Testing Data

Related Product Information for CLIC2 elisa kit

Intended Uses: This CLIC2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CLIC2. This ELISA kit for research use only!

Principle of the Assay: CLIC2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CLIC2 antibody and an CLIC2-HRP conjugate. The assay sample and buffer are incubated together with CLIC2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CLIC2 concentration since CLIC2 from samples and CLIC2-HRP conjugate compete for the anti-CLIC2 antibody binding site. Since the number of sites is limited, as more sites are occupied by CLIC2 from the sample, fewer sites are left to bind CLIC2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CLIC2 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for CLIC2 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 66346733
• NCBI GeneID: 1193
• NCBI Accession #: NP_001280.3
• NCBI GenBank Nucleotide #: NM_001289.5
• Molecular Weight: 28,356 Da
• NCBI Official Full Name: chloride intracellular channel protein 2
• NCBI Official Synonym Full Names: chloride intracellular channel 2
• NCBI Official Symbol: CLIC2
• NCBI Official Synonym Symbols: CLIC2b; MRXS32; XAP121
• NCBI Protein Information: chloride intracellular channel protein 2
• UniProt Protein Name: Chloride intracellular channel protein 2
• Protein Family: Chloride intracellular channel protein
• UniProt Gene Name: CLIC2
• UniProt Entry Name: CLIC2_HUMAN

NCBI Description

This gene encodes a chloride intracellular channel protein. Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. This protein may play a role in inhibiting the function of ryanodine receptor 2. A mutation in this gene is the cause of X-linked mental retardation-32. [provided by RefSeq, Aug 2013]

Uniprot Description

CLIC2: Can insert into membranes and form chloride ion channels. Channel activity depends on the pH. Membrane insertion seems to be redox-regulated and may occur only under oxydizing conditions. Modulates the activity of RYR2 and inhibits calcium influx. Belongs to the chloride channel CLIC family.

Protein type: Membrane protein, integral; Channel, chloride

Chromosomal Location of Human Ortholog: Xq28

Cellular Component: cytoplasm; intracellular; nucleus

Molecular Function: protein binding; chloride channel activity; glutathione transferase activity; voltage-gated ion channel activity; glutathione peroxidase activity

Biological Process: transport; positive regulation of binding; signal transduction

Disease: Mental Retardation, X-linked, Syndromic 32