Human Chemokine C-C-Motif Receptor 3 (CCR3) ELISA Kit | CCR3 elisa kit

Human Chemokine C-C-Motif Receptor 3 (CCR3) ELISA Kit

Cat. Num. AAA2022742

• Gene Names: CCR3; CKR3; CD193; CMKBR3; CC-CKR-3
• Reactivity: Human
• Synonyms: Chemokine C-C-Motif Receptor 3 (CCR3); Human Chemokine C-C-Motif Receptor 3 (CCR3) ELISA Kit; CD193; CC-CKR3; C-C CKR-3; CMKBR3; CKR3; Eosinophil eotaxin receptor; CCR3 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Chemokine C-C-Motif Receptor 3 (CCR3).; No significant cross-reactivity or interference between Chemokine C-C-Motif Receptor 3 (CCR3) and analogues was observed.
• Sequence Length: 376

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 31.25-2000pg/mL
• Sensitivity: < 13.7pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Chemokine C-C-Motif Receptor 3 (CCR3) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Chemokine C-C-Motif Receptor 3 (CCR3) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CCR3 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chemokine C-C-Motif Receptor 3 (CCR3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Chemokine C-C-Motif Receptor 3 (CCR3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chemokine C-C-Motif Receptor 3 (CCR3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chemokine C-C-Motif Receptor 3 (CCR3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for CCR3 elisa kit

CD & Adhesion molecule; Tumor immunity; Infection immunity

NCBI and Uniprot Product Information

• NCBI GI #: 120660162
• NCBI GeneID: 1232
• UniProt Accession #: P51677; Q15748; Q2YDB9; Q86WD2; Q8TDP6; Q9ULY8; B3KVQ1; F5GWL6
• Molecular Weight: 43,453 Da
• NCBI Official Full Name: Chemokine (C-C motif) receptor 3
• NCBI Official Synonym Full Names: chemokine (C-C motif) receptor 3
• NCBI Official Symbol: CCR3
• NCBI Official Synonym Symbols: CKR3; CD193; CMKBR3; CC-CKR-3
• NCBI Protein Information: C-C chemokine receptor type 3
• UniProt Protein Name: C-C chemokine receptor type 3
• Protein Family: C-C chemokine receptor
• UniProt Gene Name: CCR3
• UniProt Synonym Gene Names: CMKBR3; C-C CKR-3; CC-CKR-3; CCR-3; CCR3; CKR3
• UniProt Entry Name: CCR3_HUMAN

NCBI Description

The protein encoded by this gene is a receptor for C-C type chemokines. It belongs to family 1 of the G protein-coupled receptors. This receptor binds and responds to a variety of chemokines, including eotaxin (CCL11), eotaxin-3 (CCL26), MCP-3 (CCL7), MCP-4 (CCL13), and RANTES (CCL5). It is highly expressed in eosinophils and basophils, and is also detected in TH1 and TH2 cells, as well as in airway epithelial cells. This receptor may contribute to the accumulation and activation of eosinophils and other inflammatory cells in the allergic airway. It is also known to be an entry co-receptor for HIV-1. This gene and seven other chemokine receptor genes form a chemokine receptor gene cluster on the chromosomal region 3p21. Alternatively spliced transcript variants have been described. [provided by RefSeq, Sep 2009]

Uniprot Description

CCR3: Receptor for a C-C type chemokine. Binds to eotaxin, eotaxin-3, MCP-3, MCP-4, RANTES and MIP-1 delta. Subsequently transduces a signal by increasing the intracellular calcium ions level. Alternative coreceptor with CD4 for HIV-1 infection. Belongs to the G-protein coupled receptor 1 family.

Protein type: Motility/polarity/chemotaxis; Membrane protein, multi-pass; GPCR, family 1; Membrane protein, integral; Receptor, GPCR; Receptor, cytokine

Chromosomal Location of Human Ortholog: 3p21.3

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: protein binding; C-C chemokine receptor activity; chemokine receptor activity

Biological Process: G-protein signaling, coupled to cAMP nucleotide second messenger; positive regulation of angiogenesis; elevation of cytosolic calcium ion concentration; viral reproduction; cellular defense response; positive regulation of endothelial cell proliferation; cell adhesion; inflammatory response; chemotaxis