Human chemerin ELISA Kit | RARRES2 elisa kit

Human chemerin ELISA Kit

Cat. Num. AAA164885

• Gene Names: RARRES2; TIG2
• Reactivity: Human
• Synonyms: chemerin; Human chemerin ELISA Kit; RARRES2 elisa kit

• Reactivity: Human
• Sequence Length: 162

• Samples: Serum, plasma, cell culture supernates, cell lysates, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 10ng/L - 3000ng/L
• Sensitivity: 4.99ng/L
• Intra-Assay: CV<8%
• Inter-Assay: CV<10%
• Preparation and Storage: Store at 2 to 8 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for RARRES2 elisa kit

Intended Uses: This sandwich kit is for the accurate quantitative detection of human Chemerin (also known as Chemerin) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human Chemerin antibody. Chemerin present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human Chemerin Antibody is added and binds to Chemerin in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated Chemerin antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human Chemerin. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

NCBI and Uniprot Product Information

• NCBI GI #: 222154072
• NCBI GeneID: 508990
• Molecular Weight: 18,357 Da
• NCBI Official Full Name: chemerin
• NCBI Official Synonym Full Names: retinoic acid receptor responder 2
• NCBI Official Symbol: RARRES2
• NCBI Official Synonym Symbols: TIG2
• NCBI Protein Information: retinoic acid receptor responder protein 2
• UniProt Protein Name: Retinoic acid receptor responder protein 2
• Protein Family: Retinoic acid receptor responder protein
• UniProt Gene Name: RARRES2

Uniprot Description

Adipocyte-secreted protein (adipokine) that regulates adipogenesis, metabolism and inflammation through activation of the chemokine-like receptor 1 (CMKLR1). Its other ligands include G protein-coupled receptor 1 (GPR1) and chemokine receptor-like 2 (CCRL2). Positively regulates adipocyte differentiation, modulates the expression of adipocyte genes involved in lipid and glucose metabolism and might play a role in angiogenesis, a process essential for the expansion of white adipose tissue. Also acts as a proinflammatory adipokine, causing an increase in secretion of proinflammatory and prodiabetic adipokines, which further impair adipose tissue metabolic function and have negative systemic effects including impaired insulin sensitivity, altered glucose and lipid metabolism, and a decrease in vascular function in other tissues. Can have both pro- and anti-inflammatory properties depending on the modality of enzymatic cleavage by different classes of proteases. Acts as a chemotactic factor for leukocyte populations expressing CMKLR1, particularly immature plasmacytoid dendritic cells, but also immature myeloid DCs, macrophages and natural killer cells. Exerts an anti-inflammatory role by preventing TNF/TNFA-induced VCAM1 expression and monocytes adhesion in vascular endothelial cells. The effect is mediated via inhibiting activation of NF-kappa-B and CRK/p38 through stimulation of AKT1/NOS3 signaling and nitric oxide production. Exhibits an antimicrobial function in the skin ().