Human Centromere Protein F (CENPF) ELISA Kit | CENPF elisa kit

Human Centromere Protein F (CENPF) ELISA Kit

Cat. Num. AAA2023211

• Gene Names: CENPF; CENF; hcp-1; CILD31; PRO1779
• Reactivity: Human
• Synonyms: Centromere Protein F (CENPF); Human Centromere Protein F (CENPF) ELISA Kit; CENP-F; CENF; Hcp-1; Centromere protein F(350/400kD; Mitosin); AH antigen; Kinetochore protein CENPF; Mitosin; CENPF elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Centromere Protein F (CENPF).; No significant cross-reactivity or interference between Centromere Protein F (CENPF) and analogues was observed.
• Sequence Length: 3,210

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.056ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Centromere Protein F (CENPF) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Centromere Protein F (CENPF) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CENPF elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Centromere Protein F (CENPF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Centromere Protein F (CENPF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Centromere Protein F (CENPF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Centromere Protein F (CENPF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for CENPF elisa kit

Tumor immunity

NCBI and Uniprot Product Information

• NCBI GI #: 55770834
• NCBI GeneID: 1063
• NCBI Accession #: NP_057427.3
• NCBI GenBank Nucleotide #: NM_016343.3
• UniProt Accession #: P49454; Q13171; Q13246; Q5VVM7
• Molecular Weight: 367,764 Da
• NCBI Official Full Name: centromere protein F
• NCBI Official Synonym Full Names: centromere protein F, 350/400kDa
• NCBI Official Symbol: CENPF
• NCBI Official Synonym Symbols: CENF; hcp-1; CILD31; PRO1779
• NCBI Protein Information: centromere protein F
• UniProt Protein Name: Centromere protein F
• Protein Family: Centromere protein
• UniProt Gene Name: CENPF
• UniProt Synonym Gene Names: CENP-F
• UniProt Entry Name: CENPF_HUMAN

NCBI Description

This gene encodes a protein that associates with the centromere-kinetochore complex. The protein is a component of the nuclear matrix during the G2 phase of interphase. In late G2 the protein associates with the kinetochore and maintains this association through early anaphase. It localizes to the spindle midzone and the intracellular bridge in late anaphase and telophase, respectively, and is thought to be subsequently degraded. The localization of this protein suggests that it may play a role in chromosome segregation during mitotis. It is thought to form either a homodimer or heterodimer. Autoantibodies against this protein have been found in patients with cancer or graft versus host disease. [provided by RefSeq, Jul 2008]

Uniprot Description

CENPF: a nuclear matrix protein that assembles onto kinetochores at late G2. Is hyperphosphorylated during mitosis and rapidly degraded after mitosis. Involved in chromosome segregation during mitosis. Interacts with retinoblastoma protein (RB), CENP-E and BUBR1. Two alternatively spliced human isoforms have been described.

Protein type: Cell cycle regulation; Transcription, coactivator/corepressor; Nuclear envelope

Chromosomal Location of Human Ortholog: 1q41

Cellular Component: spindle pole; nuclear matrix; pronucleus; nuclear envelope; cytosol; kinetochore; nucleoplasm; perinuclear region of cytoplasm; cytoplasm; spindle; outer kinetochore of condensed chromosome; midbody; nucleus; chromatin; chromosome, pericentric region

Molecular Function: protein C-terminus binding; dynein binding; protein binding; protein homodimerization activity; chromatin binding; transcription factor binding

Biological Process: response to drug; mitosis; kinetochore assembly; muscle development; regulation of cell cycle; chromosome segregation; cell proliferation; protein transport; cell division; regulation of G2/M transition of mitotic cell cycle; mitotic cell cycle spindle assembly checkpoint; metaphase plate congression; mitotic cell cycle; G2/M transition of mitotic cell cycle; cell differentiation; negative regulation of transcription, DNA-dependent; regulation of striated muscle development

Disease: Ciliary Dyskinesia, Primary, 31