Human Cell Division Cycle Protein 42 (CDC42) ELISA Kit | CDC42 elisa kit

Human Cell Division Cycle Protein 42 (CDC42) ELISA Kit

Cat. Num. AAA454989

• Gene Names: CDC42; TKS; G25K; CDC42Hs
• Reactivity: Human
• Synonyms: Cell Division Cycle Protein 42 (CDC42); Human Cell Division Cycle Protein 42 (CDC42) ELISA Kit; CDC42 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of CDC42. No significant cross-reactivity or interference between CDC42 and analogues was observed.
• Sequence Length: 191

• Samples: Tissue homogenates, cell lysates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.053ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CDC42 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CDC42 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CDC42 elisa kit

Intended Uses: This kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CDC42 in human tissue homogenates, cell lysates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CDC42. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to CDC42. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CDC42, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of CDC42 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 89903012
• NCBI GeneID: 998
• NCBI Accession #: NP_001034891.1
• NCBI GenBank Nucleotide #: NM_001039802.1
• UniProt Accession #: P60953; P21181; P25763; Q7L8R5; Q9UDI2
• Molecular Weight: 21,311 Da
• NCBI Official Full Name: cell division control protein 42 homolog isoform 1
• NCBI Official Synonym Full Names: cell division cycle 42
• NCBI Official Symbol: CDC42
• NCBI Official Synonym Symbols: TKS; G25K; CDC42Hs
• NCBI Protein Information: cell division control protein 42 homolog
• UniProt Protein Name: Cell division control protein 42 homolog
• Protein Family: Cdc42
• UniProt Gene Name: CDC42

NCBI Description

The protein encoded by this gene is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. This protein is highly similar to Saccharomyces cerevisiae Cdc 42, and is able to complement the yeast cdc42-1 mutant. The product of oncogene Dbl was reported to specifically catalyze the dissociation of GDP from this protein. This protein could regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein (N-WASP), which subsequently activates Arp2/3 complex. Alternative splicing of this gene results in multiple transcript variants. Pseudogenes of this gene have been identified on chromosomes 3, 4, 5, 7, 8 and 20. [provided by RefSeq, Apr 2013]

Uniprot Description

Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. Regulates the bipolar attachment of spindle microtubules to kinetochores before chromosome congression in metaphase. Plays a role in the extension and maintenance of the formation of thin, actin-rich surface projections called filopodia. Mediates CDC42-dependent cell migration.