Human Cell division cycle 5 like protein (CDC5L) ELISA Kit | CDC5L elisa kit

Human Cell division cycle 5 like protein (CDC5L) ELISA Kit

Cat. Num. AAA7227993

• Gene Names: CDC5L; CDC5; CEF1; PCDC5RP; CDC5-LIKE; dJ319D22.1
• Reactivity: Human
• Synonyms: Cell division cycle 5 like protein (CDC5L); Human Cell division cycle 5 like protein (CDC5L) ELISA Kit; CDC5L elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of CDC5L. No significant cross-reactivity or interference between CDC5L and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CDC5L and all the analogues, therefore, cross reaction may still exist in some cases.

• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Assay Type: Quantitative Competitive
• Sensitivity: 0.1 ng/mL
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CDC5L elisa kit

Intended Uses: This CDC5L ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of HumanCDC5L. This ELISA kit for research use only, not for therapeutic or test applications!

Principle of the Assay: CDC5L ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CDC5L antibody and an CDC5L-HRP conjugate. The assay sample and buffer are incubated together with CDC5L-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CDC5L concentration since CDC5L from samples and CDC5L-HRP conjugate compete for the anti-CDC5L antibody binding site. Since the number of sites is limited, as more sites are occupied by CDC5L from the sample, fewer sites are left to bind CDC5L-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CDC5L concentration in each sample is interpolated from this standard curve.

Product Categories/Family for CDC5L elisa kit

Cell Biology

NCBI and Uniprot Product Information

• NCBI GI #: 11067747
• NCBI GeneID: 988
• NCBI Accession #: NP_001244.1
• NCBI GenBank Nucleotide #: NM_001253.3
• Molecular Weight: 92,251 Da
• NCBI Official Full Name: cell division cycle 5-like protein
• NCBI Official Synonym Full Names: cell division cycle 5-like
• NCBI Official Symbol: CDC5L
• NCBI Official Synonym Symbols: CDC5; CEF1; PCDC5RP; CDC5-LIKE; dJ319D22.1
• NCBI Protein Information: cell division cycle 5-like protein; Cdc5-related protein; pombe cdc5-related protein; dJ319D22.1 (CDC5-like protein); CDC5 cell division cycle 5-like
• UniProt Protein Name: Cell division cycle 5-like protein
• Protein Family: Cell division cycle 5-like protein
• UniProt Gene Name: CDC5L
• UniProt Synonym Gene Names: KIAA0432; PCDC5RP; Cdc5-like protein
• UniProt Entry Name: CDC5L_HUMAN

NCBI Description

The protein encoded by this gene shares a significant similarity with Schizosaccharomyces pombe cdc5 gene product, which is a cell cycle regulator important for G2/M transition. This protein has been demonstrated to act as a positive regulator of cell cycle G2/M progression. It was also found to be an essential component of a non-snRNA spliceosome, which contains at least five additional protein factors and is required for the second catalytic step of pre-mRNA splicing. [provided by RefSeq, Jul 2008]

Uniprot Description

CDC5L: is a spliceosome protein that binds DNA and that also regulates transcription. Is a positive regulator of cell cycle G2/M progression. Is an essential component of non-snRNA spliceosomes and is required for the second catalytic step of pre-mRNA splicing. Binds adeno-pre-mRNA in an ATP-stimulated manner. Is part of a spliceosomal 'core' complex that includes CDC5L, SF2, PRLI, SPF27, HSP7C, PRPF19/SNEV, GCN1L. Identified in the spliceosome C complex. Has been associated with aneuploidy in aging cells and in tumorigenesis. The genomic region containing the gene for CDC5L (6p21.1) is frequently amplified in osteosarcomas.

Protein type: Spliceosome; Motility/polarity/chemotaxis; RNA processing; Transcription factor; RNA splicing; DNA-binding

Chromosomal Location of Human Ortholog: 6p21

Cellular Component: nucleoplasm; DNA replication factor A complex; membrane; cytoplasm; nucleolus; nuclear speck; nucleus

Molecular Function: protein binding; DNA binding; chromatin binding

Biological Process: nuclear mRNA splicing, via spliceosome; transcription, DNA-dependent; regulation of transcription, DNA-dependent; RNA splicing; gene expression; DNA repair