Typical Testing Data/Standard Curve (for reference only)
Human Cell Adhesion Molecule 1 (CADM1) ELISA Kit | CADM1 elisa kit
Human Cell Adhesion Molecule 1 (CADM1) ELISA Kit
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to CADM1. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to CADM1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CADM1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of CADM1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
Uniprot Description
CADM1: a single-pass type I membrane protein that ediates homophilic cell-cell adhesion, and heterophilic cell-cell adhesion with CADM3 and PVRL3 in a Ca(2+)-independent manner. Acts as a tumor suppressor in non-small-cell lung cancer (NSCLC) cells. Interaction with CRTAM promotes natural killer (NK) cell cytotoxicity and interferon-gamma (IFN-gamma) secretion by CD8+ cells in vitro as well as NK cell-mediated rejection of tumors expressing CADM3 in vivo. May contribute to the less invasive phenotypes of lepidic growth tumor cells. In mast cells, may mediate attachment to and promote communication with nerves. CADM1, together with MITF, is essential for development and survival of mast cells in vivo. May act as a synaptic cell adhesion molecule that drives synapse assembly. May be involved in neuronal migration, axon growth, pathfinding, and fasciculation on the axons of differentiating neurons. May play diverse roles in the spermatogenesis including in the adhesion of spermatocytes and spermatids to Sertoli cells and for their normal differentiation into mature spermatozoa. Belongs to the nectin family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Vesicle; Membrane protein, integral; Tumor suppressor; Cell adhesion; Apoptosis
Chromosomal Location of Human Ortholog: 11q23.2
Cellular Component: synaptic vesicle; axon; basolateral plasma membrane; dendrite; plasma membrane; integral to membrane; intercellular junction
Molecular Function: protein binding; protein homodimerization activity; cell adhesion molecule binding; receptor binding; PDZ domain binding
Biological Process: intercellular junction assembly and maintenance; unidimensional cell growth; apoptosis; activated T cell proliferation; cell recognition; susceptibility to natural killer cell mediated cytotoxicity; liver development; positive regulation of natural killer cell mediated cytotoxicity; calcium-independent cell-cell adhesion; heterophilic cell adhesion; synaptogenesis; detection of stimulus; spermatogenesis; brain development; cell differentiation; homophilic cell adhesion; positive regulation of cytokine secretion; T cell mediated cytotoxicity
Research Articles on CADM1
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Product Notes
The Human CADM1 cadm1 (Catalog #AAA2023198) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2023198 ELISA Kit recognizes Human CADM1. It is sometimes possible for the material contained within the vial of "Cell Adhesion Molecule 1 (CADM1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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