Human CD40 ELISA Kit | CD40 elisa kit

Human CD40 ELISA Kit

Cat. Num. AAA721950

• Reactivity: Human
• Synonyms: CD40; Human CD40 ELISA Kit; CD40 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of CD40. No significant cross-reactivity or interference between CD40 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CD40 and all the analogues, therefore, cross reaction may still exist in some cases.

• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Assay Type: Quantitative Competitive
• Sensitivity: 1.0 pg/mL
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CD40 elisa kit

Intended Uses: This CD40 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CD40. This ELISA kit for research use only, not for therapeutic or test applications!

Principle of the Assay: CD40 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-CD40 antibody and an CD40-HRP conjugate. The assay sample and buffer are incubated together with CD40-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CD40 concentration since CD40 from samples and CD40-HRP conjugate compete for the anti-CD40 antibody binding site. Since the number of sites is limited, as more sites are occupied by CD40 from the sample, fewer sites are left to bind CD40-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CD40 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for CD40 elisa kit

Immunology

NCBI and Uniprot Product Information

• NCBI GI #: 114053977
• Molecular Weight: 13,158 Da
• NCBI Official Full Name: CD40, partial
• UniProt Protein Name: CD40
• Protein Family: CD40 ligand
• UniProt Entry Name: Q09LL4_HUMAN

NCBI Description

This gene is a member of the TNF-receptor superfamily. The encoded protein is a receptor on antigen-presenting cells of the immune system and is essential for mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of this receptor and its ligand, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of this receptor and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. Mutations affecting this gene are the cause of autosomal recessive hyper-IgM immunodeficiency type 3 (HIGM3). Multiple alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Nov 2014]

Uniprot Description

CD40: a member of the TNF-receptor superfamily. This receptor for CD40L mediates a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. Defects in CD40 are the cause of hyper-IgM immunodeficiency type 3 (HIGM3). HIGM3 is an autosomal recessive disorder which includes an inability of B cells to undergo isotype switching, one of the final differentiation steps in the humoral immune system, an inability to mount an antibody-specific immune response, and a lack of germinal center formation. Two alternatively spliced isoforms have been reported. Isoform I is a type I membrane protein; isoform II is secreted.

Protein type: Receptor, cytokine; Membrane protein, integral

Chromosomal Location of Human Ortholog: 20q12-q13.2

Cellular Component: extracellular space; cell surface; intracellular membrane-bound organelle; integral to plasma membrane; cytoplasm; plasma membrane; external side of plasma membrane

Molecular Function: protein binding; signal transducer activity; enzyme binding; ubiquitin protein ligase binding; receptor activity; antigen binding

Biological Process: B cell proliferation; platelet activation; positive regulation of isotype switching to IgG isotypes; regulation of immune response; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of interleukin-12 production; immune response-regulating cell surface receptor signaling pathway; activation of NF-kappaB transcription factor; cellular calcium ion homeostasis; positive regulation of MAP kinase activity; positive regulation of tyrosine phosphorylation of Stat1 protein; positive regulation of B cell proliferation; protein kinase B signaling cascade; positive regulation of transcription from RNA polymerase II promoter; protein complex assembly; positive regulation of protein amino acid phosphorylation; inflammatory response; regulation of immunoglobulin secretion; defense response to virus

Disease: Immunodeficiency With Hyper-igm, Type 3