CD300A elisa kit

Human CD300A PicoKine ELISA Kit

Cat. Num. AAA1751721

• Gene Names: CD300A; IRC1; IRC2; CLM-8; IRp60; IGSF12; CMRF35H; CMRF-35H; CMRF35-H; CMRF35H9; CMRF35-H9; IRC1/IRC2; CMRF-35-H9
• Reactivity: Human; There is no detectable cross-reactivity with other relevant proteins.
• Synonyms: CD300A; Human CD300A PicoKine ELISA Kit; Human CD300A; CMRF35-like molecule 8; CLM-8; CD300 antigen-like family member A; CMRF-35-H9; CMRF35-H9; CMRF35-H; IRC1/IRC2; Immunoglobulin superfamily member 12; IgSF12; Inhibitory receptor protein 60; IRp60; NK inhibitory receptor; CD300a; CMRF35H; IGSF12; HSPC083; CD300a molecule; CD300A elisa kit

• Reactivity: Human; There is no detectable cross-reactivity with other relevant proteins.
• Specificity: Natural and recombinant Human CD200
• Sequence Length: 186

• Samples: Cell Culture Supernates, Cell Lysates, Serum And Plasma (Heparin, Edta)
• Detection Range: 31.2pg/ml-2000pg/ml
• Sensitivity: <10pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision
• Inter-assay Precision: Inter-Assay Precision (Precision accross assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months. Store at -20 degree C for 12 months.; Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CD300A elisa kit

Principle of the Assay: The Picokine Human CD200 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human CD200 with a 96-well strip plate that is pre-coated with antibody specific for CD200. The detection antibody is a biotinylated antibody specific for CD200. The capture antibody is monoclonal antibody from mouse, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Human CD200 with immunogen: Expression system for standard: NSO; Immunogen sequence: Q31-G232. The kit is analytically validated with ready to use reagents. To measure Human CD200, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly propotional to Human CD200 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human CD200 in the sample!!Background/Introduction: OX-2 membrane glycoprotein, also known as CD200 is a human protein encoded by the CD200 gene. This gene is mapped to 3q13.2. The protein encoded by this gene is a type-1 membrane glycoprotein, which contains two immunoglobulin domains, and thus belongs to the immunoglobulin superfamily. Studies of the related genes in mouse and rat suggest that this gene may regulate myeloid cell activity and delivers an inhibitory signal for the macrophage lineage in diverse tissues. It is suggested that CD200-CD200R signaling plays a central role in macrophage fusion and osteoclast formation.

NCBI and Uniprot Product Information

• NCBI GI #: 378786683
• NCBI GeneID: 11314
• NCBI Accession #: NP_001243770.1
• NCBI GenBank Nucleotide #: NM_001256841.1
• UniProt Accession #: Q9UGN4; O95100; Q9HD97; Q9P0F3; Q9UBK4; Q9UMS9; Q9UMT0; A8MW96
• Molecular Weight: 20,640 Da
• NCBI Official Full Name: CMRF35-like molecule 8 isoform 2
• NCBI Official Synonym Full Names: CD300a molecule
• NCBI Official Symbol: CD300A
• NCBI Official Synonym Symbols: IRC1; IRC2; CLM-8; IRp60; IGSF12; CMRF35H; CMRF-35H; CMRF35-H; CMRF35H9; CMRF35-H9; IRC1/IRC2; CMRF-35-H9
• NCBI Protein Information: CMRF35-like molecule 8
• UniProt Protein Name: CMRF35-like molecule 8
• Protein Family: CMRF35-like molecule
• UniProt Gene Name: CD300A
• UniProt Synonym Gene Names: CMRF35H; IGSF12; CLM-8; CMRF35-H9; IgSF12; IRp60

NCBI Description

This gene encodes a member of the CD300 glycoprotein family of cell surface proteins found on leukocytes involved in immune response signaling pathways. This gene is located on chromosome 17 in a cluster with all but one of the other family members. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2012]

Uniprot Description

Inhibitory receptor which may contribute to the down-regulation of cytolytic activity in natural killer (NK) cells, and to the down-regulation of mast cell degranulation (PubMed:10746781, PubMed:16339535, PubMed:9701027). Negatively regulates the Toll-like receptor (TLR) signaling mediated by MYD88 but not TRIF through activation of PTPN6 (PubMed:22043923).