CD25 elisa kit
Human CD25 ELISA Kit
Reactivity
Human. No cross reactivity with other human soluble receptors.
Synonyms
CD25; Human CD25 ELISA Kit; CD25 / IL-2R; CD25 elisa kit
Reactivity
Human. No cross reactivity with other human soluble receptors.
Specificity
Recognizes both natural and recombinant human soluble IL-2R
Sequence Length
160
Samples
Supernatants, buffered solutions or serum and plasma samples
Sensitivity
The sensitivity or minimum detectable dose of CD25 using this CD25 ELISA kit was found to be 32.5pg/ml. This was determined by adding 2 standard deviations to the mean OD obtained when the zero standard was assayed in 6 independent experiments.
Intra-assay Precision
Reproducibility within the assay will be evaluated in three independent experiments. Each assay will be carried out with 6 replicates (3 duplicates) in 2 human pooled serum, 2 in RPMI and 2 in standard diluent with samples containing different concentrations of CD25. 2 standard curves were run on each plate The overall intra-assay coefficient of variation has been calculated to be 4.5%.
Inter-assay Precision
Assay to assay reproducibility within one laboratory will be evaluated in three independent experiments by two technicians. Each assay will be carried out with 6 replicates (3 duplicates) in 2 human pooled serum, 2 in RPMI and 2 in standard diluent with samples containing different concentrations of CD25. 2 standard curves were run on each plate. The calculated overall coefficient of variation was 10.3%.
Intended Uses
The sCD25 ELISA kit is a solid phase sandwich ELISA for the in-vitro qualitative and quantitative determination of sCD25 in supernatants, buffered solutions or serum and plasma samples. This assay will recognise both natural and recombinant human sCD25.
Related Product Information for CD25 elisa kit
MyBioSource ELISA Kits include pre-coated strip plates, biotinylated secondary antibody, standards, controls (where applicable), buffers, streptavidin-HRP, TMB, stop reagents, and a detailed procedure.
Product Categories/Family for CD25 elisa kit
References
Botella-Carretero J. I. et al., Eur. J. Endocrinol., 2005; 153(2): 223 - 230
Dennert, R. et al., Clin. Vaccine Immunol.,2012; 19(8): 1182-1187
Foussat A. et al., Blood, 2001; 98(6): 1678 - 1686
Dennert, R. et al., Clin. Vaccine Immunol.,2012; 19(8): 1182-1187
Foussat A. et al., Blood, 2001; 98(6): 1678 - 1686
NCBI and Uniprot Product Information
Uniprot Description
Might be involved in growth regulation, and in myelinization in the peripheral nervous system.
Similar Products
Product Notes
The Human. No cross reactivity with other human soluble receptors. CD25 pmp22 (Catalog #AAA335497) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA335497 ELISA Kit recognizes Human. No cross reactivity with other human soluble receptors. CD25. It is sometimes possible for the material contained within the vial of "CD25, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
