Human CD200 Receptor 1 (CD200R1) ELISA Kit | CD200R1 elisa kit

Human CD200 Receptor 1 (CD200R1) ELISA Kit

Cat. Num. AAA2707122

• Gene Names: CD200R1; OX2R; MOX2R; CD200R; HCRTR2
• Reactivity: Human
• Synonyms: CD200 Receptor 1 (CD200R1); Human CD200 Receptor 1 (CD200R1) ELISA Kit; OX2R; HCRTR2; CD200R; MOX2R; MOX2 Receptor; Cell surface glycoprotein CD200 receptor 1; CD200 cell surface glycoprotein receptor; Cell surface glycoprotein OX2 receptor 1; CD200R1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of CD200 Receptor 1 (CD200R1).; No significant cross-reactivity or interference between CD200 Receptor 1 (CD200R1) and analogues was observed.
• Sequence Length: 348

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 15.62-1000pg/mL
• Sensitivity: < 6.6pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CD200 Receptor 1 (CD200R1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CD200 Receptor 1 (CD200R1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CD200R1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to CD200 Receptor 1 (CD200R1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to CD200 Receptor 1 (CD200R1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CD200 Receptor 1 (CD200R1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of CD200 Receptor 1 (CD200R1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 20270365
• NCBI GeneID: 131450
• NCBI Accession #: NP_620161.1
• NCBI GenBank Nucleotide #: NM_138806.3
• UniProt Accession #: Q8TD46; Q52LJ7; Q6IS95; Q6UW94; Q6WHB8; Q8TD44; Q8TD45; Q8TD52; B3KWZ9; E9PCM9
• Molecular Weight: 39,056 Da
• NCBI Official Full Name: cell surface glycoprotein CD200 receptor 1 isoform a
• NCBI Official Synonym Full Names: CD200 receptor 1
• NCBI Official Symbol: CD200R1
• NCBI Official Synonym Symbols: OX2R; MOX2R; CD200R; HCRTR2
• NCBI Protein Information: cell surface glycoprotein CD200 receptor 1
• UniProt Protein Name: Cell surface glycoprotein CD200 receptor 1
• Protein Family: Cell surface glycoprotein CD200 receptor
• UniProt Gene Name: CD200R1
• UniProt Synonym Gene Names: CD200R; CRTR2; MOX2R; OX2R

NCBI Description

This gene encodes a receptor for the OX-2 membrane glycoprotein. Both the receptor and substrate are cell surface glycoproteins containing two immunoglobulin-like domains. This receptor is restricted to the surfaces of myeloid lineage cells and the receptor-substrate interaction may function as a myeloid downregulatory signal. Mouse studies of a related gene suggest that this interaction may control myeloid function in a tissue-specific manner. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jul 2008]

Uniprot Description

Inhibitory receptor for the CD200/OX2 cell surface glycoprotein. Limits inflammation by inhibiting the expression of proinflammatory molecules including TNF-alpha, interferons, and inducible nitric oxide synthase (iNOS) in response to selected stimuli. Also binds to HHV-8 K14 viral CD200 homolog with identical affinity and kinetics as the host CD200.