Typical Testing Data/Standard Curve (for reference only)
Human CCR2 ELISA Kit | CCR2 elisa kit
Human CCR2 (Chemokine C-C-Motif Receptor 2) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CCR2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CCR2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CCR2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human CCR2. You can calculate the concentration of Human CCR2 in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes two isoforms of a receptor for monocyte chemoattractant protein-1, a chemokine which specifically mediates monocyte chemotaxis. Monocyte chemoattractant protein-1 is involved in monocyte infiltration in inflammatory diseases such as rheumatoid arthritis as well as in the inflammatory response against tumors. The receptors encoded by this gene mediate agonist-dependent calcium mobilization and inhibition of adenylyl cyclase. This gene is located in the chemokine receptor gene cluster region. Two alternatively spliced transcript variants are expressed by the gene. [provided by RefSeq, Mar 2009]
Uniprot Description
CCR2: a seven transmembrane protein closely related to CCR1. Receptor for the MCP-1, MCP-3 and MCP-4 chemokines. Expressed at high levels in primary neutrophils and primary monocytes, and is further upregulated on neutrophil activation and during monocyte to macrophage differentiation. Transduces a signal by increasing the intracellular calcium ions level. Alternative co receptor with CD4 for HIV-1 infection. Two splice-variant isoforms have been described.
Protein type: Membrane protein, multi-pass; Membrane protein, integral; GPCR, family 1; Receptor, GPCR
Chromosomal Location of Human Ortholog: 3p21.31
Cellular Component: cell soma; integral to plasma membrane; perinuclear region of cytoplasm; dendrite; cytoplasm; integral to membrane; plasma membrane; perikaryon; cytosol
Molecular Function: protein homodimerization activity; CCR2 chemokine receptor binding; C-C chemokine receptor activity; chemokine receptor activity
Biological Process: positive regulation of T-helper 1 type immune response; viral reproduction; chemotaxis; elevation of cytosolic calcium ion concentration; response to wounding; dendritic cell chemotaxis; cellular homeostasis; inflammatory response; positive regulation of tumor necrosis factor biosynthetic process; cytokine and chemokine mediated signaling pathway; negative regulation of adenylate cyclase activity; JAK-STAT cascade; cellular calcium ion homeostasis; G-protein coupled receptor protein signaling pathway; negative regulation of angiogenesis; positive regulation of interferon-gamma production; negative regulation of T-helper 2 type immune response; positive regulation of interleukin-2 production; innate immune response; cellular defense response; blood vessel remodeling; positive regulation of alpha-beta T cell proliferation; immune response; positive regulation of T cell activation; negative regulation of eosinophil degranulation; positive regulation of inflammatory response
Research Articles on CCR2
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Product Notes
The Human CCR2 ccr2 (Catalog #AAA2516082) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2516082 ELISA Kit recognizes Human CCR2. It is sometimes possible for the material contained within the vial of "CCR2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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