Typical Testing Data/Standard Curve (for reference only)
Human Cation channel sperm associated protein 4 (CATSPER4) ELISA Kit | CATSPER4 elisa kit
Human Cation channel sperm associated protein 4 (CATSPER4) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: CATSPER4 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-CATSPER4 antibody and an CATSPER4-HRP conjugate. The assay sample and buffer are incubated together with CATSPER4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CATSPER4 concentration since CATSPER4 from samples and CATSPER4-HRP conjugate compete for the anti-CATSPER4 antibody binding site. Since the number of sites is limited, as more sites are occupied by CATSPER4 from the sample, fewer sites are left to bind CATSPER4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CATSPER4 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
Uniprot Description
CATSPER4: Voltage-gated calcium channel that plays a central role in calcium-dependent physiological responses essential for successful fertilization, such as sperm hyperactivation, acrosome reaction and chemotaxis towards the oocyte. Activated by extracellular progesterone and prostaglandins following the sequence: progesterone > PGF1-alpha = PGE1 > PGA1 > PGE2 >> PGD2. The primary effect of progesterone activation is to shift voltage dependence towards more physiological, negative membrane potentials; it is not mediated by metabotropic receptors and second messengers. Sperm capacitation enhances the effect of progesterone by providing additional negative shift. Also activated by the elevation of intracellular pH. Belongs to the cation channel sperm-associated (TC 1.A.1.19) family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; Membrane protein, multi-pass
Chromosomal Location of Human Ortholog: 1p35.3
Cellular Component: plasma membrane; acrosome
Molecular Function: voltage-gated calcium channel activity; calcium activated cation channel activity
Biological Process: sperm-egg recognition; sperm motility; multicellular organismal development; single fertilization; sodium ion transport; sperm capacitation; multicellular organism reproduction
Research Articles on CATSPER4
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Product Notes
The Human CATSPER4 catsper4 (Catalog #AAA7211132) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7211132 ELISA Kit recognizes Human CATSPER4. It is sometimes possible for the material contained within the vial of "Cation channel sperm associated protein 4 (CATSPER4), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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