Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Cathepsin Z (CTSZ) Standard Curve.)
Human Cathepsin Z (CTSZ) ELISA Kit | CTSZ elisa kit
Human Cathepsin Z (CTSZ) ELISA Kit
Gene Names
CTSZ; CTSX
Reactivity
Human
Synonyms
Cathepsin Z (CTSZ); Human Cathepsin Z (CTSZ) ELISA Kit; CTSZ; CTSX; FLJ17088; cathepsin X; preprocathepsin P; CTSZ elisa kit
Reactivity
Human
Specificity
Human Cathepsin Z (CTSZ) ELISA Kit has high sensitivity and excellent specificity for detection of Human CTSZ. No significant cross-reactivity or interference between Human CTSZ and analogues was observed.
Sequence Length
303
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Cathepsin Z (CTSZ) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Cathepsin Z (CTSZ) Standard Curve.)
Related Product Information for CTSZ elisa kit
Description: This Human Cathepsin Z (CTSZ) ELISA Kit employs a two-site sandwich ELISA to quantitate CTSZ in samples. An antibody specific for CTSZ has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CTSZ present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CTSZ is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CTSZ bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Cathepsin Z or cathepsin X is a lysosomal cysteine proteinase and member of the peptidase C1 family. It exhibits both carboxy-monopeptidase and carboxy-dipeptidase activities. The encoded protein has also been known as cathepsin X and cathepsin P. This gene is expressed ubiquitously in cancer cell lines and primary tumors and, like other members of this family, may be involved in tumorigenesis. The predicted 303-amino acid protein has the domain structure characteristic of cysteine proteinases, including a signal sequence, a prodomain, and a catalytic domain responsible for proteolytic activity. However, it also contains 3 unique peptide insertions. Cathepsin Z shares protein sequence identity with other human cysteine proteinases of the papain family, including 34% with cathepsin C and 26% with cathepsin B.
Background: Cathepsin Z or cathepsin X is a lysosomal cysteine proteinase and member of the peptidase C1 family. It exhibits both carboxy-monopeptidase and carboxy-dipeptidase activities. The encoded protein has also been known as cathepsin X and cathepsin P. This gene is expressed ubiquitously in cancer cell lines and primary tumors and, like other members of this family, may be involved in tumorigenesis. The predicted 303-amino acid protein has the domain structure characteristic of cysteine proteinases, including a signal sequence, a prodomain, and a catalytic domain responsible for proteolytic activity. However, it also contains 3 unique peptide insertions. Cathepsin Z shares protein sequence identity with other human cysteine proteinases of the papain family, including 34% with cathepsin C and 26% with cathepsin B.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
cathepsin Z preproprotein
NCBI Official Synonym Full Names
cathepsin Z
NCBI Official Symbol
CTSZ
NCBI Official Synonym Symbols
CTSX
NCBI Protein Information
cathepsin Z
UniProt Protein Name
Cathepsin Z
Protein Family
UniProt Gene Name
CTSZ
UniProt Entry Name
CATZ_HUMAN
NCBI Description
The protein encoded by this gene is a lysosomal cysteine proteinase and member of the peptidase C1 family. It exhibits both carboxy-monopeptidase and carboxy-dipeptidase activities. The encoded protein has also been known as cathepsin X and cathepsin P. This gene is expressed ubiquitously in cancer cell lines and primary tumors and, like other members of this family, may be involved in tumorigenesis. [provided by RefSeq, Oct 2008]
Uniprot Description
CTSZ: Exhibits carboxy-monopeptidase as well as carboxy- dipeptidase activity. Belongs to the peptidase C1 family.
Protein type: EC 3.4.18.1; Protease
Chromosomal Location of Human Ortholog: 20q13.32
Cellular Component: extracellular space; intracellular membrane-bound organelle; lysosome; endoplasmic reticulum; plasma membrane
Molecular Function: cysteine-type peptidase activity
Biological Process: cellular protein metabolic process; proteolysis; angiotensin maturation
Disease: Mycobacterium Tuberculosis, Susceptibility To
Research Articles on CTSZ
Similar Products
Product Notes
The Human CTSZ ctsz (Catalog #AAA9716077) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9716077 ELISA Kit recognizes Human CTSZ. It is sometimes possible for the material contained within the vial of "Cathepsin Z (CTSZ), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
