Human Calpain 14 (CAPN14) ELISA Kit | CAPN14 elisa kit
Human Calpain 14 (CAPN14) ELISA Kit
Reactivity
Human
Synonyms
Calpain 14 (CAPN14); Human Calpain 14 (CAPN14) ELISA Kit; CAPN14 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of CAPN14. No significant cross-reactivity or interference between CAPN14 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CAPN14 and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
0.1 ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
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Related Product Information for CAPN14 elisa kit
Intended Uses: This CAPN14 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CAPN14. This ELISA kit for research use only, not for therapeutic or test applications!
Principle of the Assay: CAPN14 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CAPN14 antibody and an CAPN14-HRP conjugate. The assay sample and buffer are incubated together with CAPN14-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CAPN14 concentration since CAPN14 from samples and CAPN14-HRP conjugate compete for the anti-CAPN14 antibody binding site. Since the number of sites is limited, as more sites are occupied by CAPN14 from the sample, fewer sites are left to bind CAPN14-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CAPN14 concentration in each sample is interpolated from this standard curve.
Principle of the Assay: CAPN14 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CAPN14 antibody and an CAPN14-HRP conjugate. The assay sample and buffer are incubated together with CAPN14-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CAPN14 concentration since CAPN14 from samples and CAPN14-HRP conjugate compete for the anti-CAPN14 antibody binding site. Since the number of sites is limited, as more sites are occupied by CAPN14 from the sample, fewer sites are left to bind CAPN14-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CAPN14 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
59,025 Da
NCBI Official Full Name
calpain-14
NCBI Official Synonym Full Names
calpain 14
NCBI Official Symbol
CAPN14
NCBI Protein Information
calpain-14; CANP 14; calcium-activated neutral proteinase 14
UniProt Protein Name
Calpain-14
Protein Family
UniProt Gene Name
CAPN14
UniProt Synonym Gene Names
CANP 14
UniProt Entry Name
CAN14_HUMAN
NCBI Description
Calpains are a family of cytosolic calcium-activated cysteine proteases involved in a variety of cellular processes including apoptosis, cell division, modulation of integrin-cytoskeletal interactions, and synaptic plasticity (Dear et al., 2000 [PubMed 10964513]). CAPN14 belongs to the calpain large subunit family.[supplied by OMIM, Mar 2008]
Uniprot Description
CAPN14 iso2: Calcium-regulated non-lysosomal thiol-protease. Belongs to the peptidase C2 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 3.4.22.-; Protease
Chromosomal Location of Human Ortholog: 2p23.1-p21
Cellular Component: nucleoplasm; cytoplasm
Molecular Function: calcium-dependent cysteine-type endopeptidase activity; calcium ion binding
Biological Process: proteolysis
