Typical Testing Data/Standard Curve (for reference only)
Human CALCRL/Calcitonin Gene-Related Peptide Type 1 Receptor ELISA Kit | CALCRL elisa kit
Human CALCRL/Calcitonin Gene-Related Peptide Type 1 Receptor ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen, During the reaction, target antigen in the sample or standard competes with a fixed amount of biotin-labeled target antigen for sites on a pre-coated antibody specific to target antigen. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
Uniprot Description
CALCRL: Receptor for calcitonin-gene-related peptide (CGRP) together with RAMP1 and receptor for adrenomedullin together with RAMP3. Receptor for adrenomedullin together with RAMP2. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. Belongs to the G-protein coupled receptor 2 family.
Protein type: Membrane protein, multi-pass; GPCR, family 2; Cell cycle regulation; Receptor, GPCR; Membrane protein, integral
Chromosomal Location of Human Ortholog: 2q32.1
Cellular Component: endoplasmic reticulum; lysosome; integral to plasma membrane; plasma membrane; endosome
Molecular Function: G-protein coupled receptor activity; protein binding; calcitonin gene-related polypeptide receptor activity; adrenomedullin receptor activity; protein transporter activity; calcitonin receptor activity
Biological Process: G-protein signaling, coupled to cyclic nucleotide second messenger; protein transport; regulation of muscle contraction; calcium ion transport; receptor internalization; positive regulation of smooth muscle cell proliferation; heart development; negative regulation of inflammatory response; positive regulation of cAMP biosynthetic process; cAMP biosynthetic process; angiogenesis; G-protein signaling, adenylate cyclase activating pathway
Research Articles on CALCRL
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Product Notes
The Human CALCRL calcrl (Catalog #AAA2889963) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2889963 ELISA Kit recognizes Human CALCRL. It is sometimes possible for the material contained within the vial of "CALCRL/Calcitonin Gene-Related Peptide Type 1 Receptor, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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