Human C-Type Lectin Domain Family 11, Member A (CLEC11A) ELISA Kit | CLEC11A elisa kit

Human C-Type Lectin Domain Family 11, Member A (CLEC11A) ELISA Kit

Cat. Num. AAA2018968

• Gene Names: CLEC11A; P47; SCGF; LSLCL; CLECSF3
• Reactivity: Human
• Synonyms: C-Type Lectin Domain Family 11; Member A (CLEC11A); Human C-Type Lectin Domain Family 11; Member A (CLEC11A) ELISA Kit; SCGF; P47; LSLCL; CLECSF3; Stem Cell Growth Factor; Lymphocyte Secreted C-Type Lectin; C-type lectin superfamily member 3; CLEC11A elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of C-Type Lectin Domain Family 11, Member A (CLEC11A).; No significant cross-reactivity or interference between C-Type Lectin Domain Family 11, Member A (CLEC11A) and analogues was observed.
• Sequence Length: 323

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates and Other Biological Fluids
• Detection Range: 0.94-60ng/mL
• Sensitivity: < 0.39ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level C-Type Lectin Domain Family 11, Member A (CLEC11A) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level C-Type Lectin Domain Family 11, Member A (CLEC11A) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CLEC11A elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to C-Type Lectin Domain Family 11, Member A (CLEC11A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to C-Type Lectin Domain Family 11, Member A (CLEC11A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain C-Type Lectin Domain Family 11, Member A (CLEC11A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of C-Type Lectin Domain Family 11, Member A (CLEC11A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for CLEC11A elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 4506803
• NCBI GeneID: 6320
• NCBI Accession #: NP_002966.1
• NCBI GenBank Nucleotide #: NM_002975.2
• UniProt Accession #: Q9Y240; B2RAD4
• Molecular Weight: 35,695 Da
• NCBI Official Full Name: C-type lectin domain family 11 member A
• NCBI Official Synonym Full Names: C-type lectin domain family 11, member A
• NCBI Official Symbol: CLEC11A
• NCBI Official Synonym Symbols: P47; SCGF; LSLCL; CLECSF3
• NCBI Protein Information: C-type lectin domain family 11 member A
• UniProt Protein Name: C-type lectin domain family 11 member A
• Protein Family: C-type lectin domain family
• UniProt Gene Name: CLEC11A
• UniProt Synonym Gene Names: CLECSF3; LSLCL; SCGF
• UniProt Entry Name: CLC11_HUMAN

NCBI Description

This gene encodes a member of the C-type lectin superfamily. The encoded protein is a secreted sulfated glycoprotein and functions as a growth factor for primitive hematopoietic progenitor cells. An alternative splice variant has been described but its biological nature has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

CLEC11A: Stimulates the proliferation and differentiation of hematopoietic precursor cells from various lineages, including erythrocytes, lymphocytes, granulocytes and macrophages. Acts synergistically with other cytokines, including IL-3, GCSF, GMCSF and FLT3 ligand. Suppresses SCF-stimulated erythrocyte proliferation.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: extracellular space; cytoplasm; extracellular region

Molecular Function: growth factor activity; carbohydrate binding

Biological Process: positive regulation of cell proliferation