Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human C-Myc-binding protein (MYCBP) Standard Curve.)
Human C-Myc-Binding Protein (MYCBP) ELISA Kit | MYCBP elisa kit
Human C-Myc-Binding Protein (MYCBP) ELISA Kit
Gene Names
MYCBP; AMY-1
Reactivity
Human
Synonyms
C-Myc-Binding Protein (MYCBP); Human C-Myc-Binding Protein (MYCBP) ELISA Kit; MYCBP; AMY-1; FLJ41056; associate of myc-1; MYCBP elisa kit
Reactivity
Human
Specificity
Human C-Myc-binding protein (MYCBP) ELISA Kit has high sensitivity and excellent specificity for detection of Human MYCBP. No significant cross-reactivity or interference between Human MYCBP and analogues was observed.
Sequence Length
103
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human C-Myc-binding protein (MYCBP) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human C-Myc-binding protein (MYCBP) Standard Curve.)
Related Product Information for MYCBP elisa kit
Description: This Human C-Myc-binding protein (MYCBP) ELISA Kit employs a two-site sandwich ELISA to quantitate MYCBP in samples. An antibody specific for MYCBP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MYCBP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MYCBP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MYCBP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The MYCBP gene encodes a protein that binds to the N-terminal region of MYC and stimulates the activation of E box-dependent transcription by MYC.The MYC protooncogene appears to play key roles in cell proliferation, differentiation, transformation, and apoptosis. A variety of functions of the MYC gene product are attributable to protein-protein interactions with various cellular factors. AMY1 was found to bind in vitro and in vivo to the regulatory subunit II-binding region of AKAP1 and S-AKAP84, a splicing variant of AKAP149 expressed in the testis. AMY1 was expressed postmeiotically in the testis, as was also S-AKAP84. AMY1 was localized in the mitochondria of HeLa and sperm in association with AKAP149 and S-AKAP84, respectively. These results suggested that AMY1 plays a role in spermatogenesis.
Background: The MYCBP gene encodes a protein that binds to the N-terminal region of MYC and stimulates the activation of E box-dependent transcription by MYC.The MYC protooncogene appears to play key roles in cell proliferation, differentiation, transformation, and apoptosis. A variety of functions of the MYC gene product are attributable to protein-protein interactions with various cellular factors. AMY1 was found to bind in vitro and in vivo to the regulatory subunit II-binding region of AKAP1 and S-AKAP84, a splicing variant of AKAP149 expressed in the testis. AMY1 was expressed postmeiotically in the testis, as was also S-AKAP84. AMY1 was localized in the mitochondria of HeLa and sperm in association with AKAP149 and S-AKAP84, respectively. These results suggested that AMY1 plays a role in spermatogenesis.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
c-Myc-binding protein
NCBI Official Synonym Full Names
MYC binding protein
NCBI Official Symbol
MYCBP
NCBI Official Synonym Symbols
AMY-1
NCBI Protein Information
C-Myc-binding protein
UniProt Protein Name
C-Myc-binding protein
Protein Family
UniProt Gene Name
MYCBP
UniProt Synonym Gene Names
AMY1; AMY-1
UniProt Entry Name
MYCBP_HUMAN
NCBI Description
The protein encoded by this gene binds to the N-terminus of the oncogenic protein C-MYC, enhancing the ability of C-MYC to activate E box-dependent transcription. The encoded protein is normally found in the cytoplasm, but it translocates to the nucleus during S phase of the cell cycle and associates with C-MYC. This protein may be involved in spermatogenesis. This gene can be silenced by microRNA-22. Two transcript variants, one protein-coding and the other probably not protein-coding, have been found for this gene. [provided by RefSeq, Nov 2011]
Uniprot Description
MYCBP: May control the transcriptional activity of MYC. Stimulates the activation of E box-dependent transcription by MYC. Belongs to the AMY1 family.
Protein type: Transcription, coactivator/corepressor
Chromosomal Location of Human Ortholog: 1p33-p32.2
Cellular Component: centrosome; mitochondrion; cytoplasm; nucleus
Molecular Function: protein binding; transcription coactivator activity
Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent; spermatogenesis
Research Articles on MYCBP
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Product Notes
The Human MYCBP mycbp (Catalog #AAA9716194) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9716194 ELISA Kit recognizes Human MYCBP. It is sometimes possible for the material contained within the vial of "C-Myc-Binding Protein (MYCBP), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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