Typical Testing Data/Standard Curve (for reference only)
Human BRISC complex subunit Abro1 (FAM175B) ELISA Kit | FAM175B elisa kit
Human BRISC complex subunit Abro1 (FAM175B) ELISA Kit
Gene Names
FAM175B; ABRO1; KIAA0157
Reactivity
Human
Synonyms
BRISC complex subunit Abro1 (FAM175B); Human BRISC complex subunit Abro1 (FAM175B) ELISA Kit; FAM175B elisa kit
Reactivity
Human
Samples
Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate
Assay Type
Quantitative Competitive
Sensitivity
0.1 ng/mL.
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for FAM175B elisa kit
Intended Uses: This FABP2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of RatFABP2. This ELISA kit for research use only!
Principle of the Assay: FABP2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-FABP2 antibody and an FABP2-HRP conjugate. The assay sample and buffer are incubated together with FABP2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FABP2 concentration since FABP2 from samples and FABP2-HRP conjugate compete for the anti-FABP2 antibody binding site. Since the number of sites is limited, as more sites are occupied by FABP2 from the sample, fewer sites are left to bind FABP2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FABP2 concentration in each sample is interpolated from this standard curve.
Principle of the Assay: FABP2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-FABP2 antibody and an FABP2-HRP conjugate. The assay sample and buffer are incubated together with FABP2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FABP2 concentration since FABP2 from samples and FABP2-HRP conjugate compete for the anti-FABP2 antibody binding site. Since the number of sites is limited, as more sites are occupied by FABP2 from the sample, fewer sites are left to bind FABP2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FABP2 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for FAM175B elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
46,901 Da
NCBI Official Full Name
BRISC complex subunit Abro1
NCBI Official Synonym Full Names
family with sequence similarity 175, member B
NCBI Official Symbol
FAM175B
NCBI Official Synonym Symbols
ABRO1; KIAA0157
NCBI Protein Information
BRISC complex subunit Abro1; abraxas brother 1; abraxas brother protein 1
UniProt Protein Name
BRISC complex subunit Abro1
Protein Family
UniProt Gene Name
FAM175B
UniProt Synonym Gene Names
ABRO1; KIAA0157
UniProt Entry Name
F175B_HUMAN
Uniprot Description
FAM175B: a protein of unknown function.
Protein type: Ubiquitin conjugating system
Chromosomal Location of Human Ortholog: 10q26.13
Cellular Component: cytoplasm
Molecular Function: polyubiquitin binding
Research Articles on FAM175B
Similar Products
Product Notes
The Human FAM175B fam175b (Catalog #AAA7236017) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7236017 ELISA Kit recognizes Human FAM175B. It is sometimes possible for the material contained within the vial of "BRISC complex subunit Abro1 (FAM175B), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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