Human BLNK ELISA Kit | BLNK elisa kit

Human BLNK (B-Cell Linker Protein) ELISA Kit

Cat. Num. AAA2506119

• Gene Names: BLNK; bca; AGM4; BASH; LY57; SLP65; BLNK-S; SLP-65
• Reactivity: Human
• Synonyms: BLNK; Human BLNK (B-Cell Linker Protein) ELISA Kit; BLNK elisa kit

• Reactivity: Human
• Specificity: This kit recognizes natural and recombinantHumanBLNK. No significant cross-reactivity or interference between HumanBLNK and analogues was observed.
• Sequence Length: 433

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: Min: 0.094ng/mL; Max: 10ng/mL
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for BLNK elisa kit

Intended Uses: This ELISA kit applies to the invitro quantitative determination of HumanBLNKconcentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to BLNK. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for BLNKand Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain BLNK, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of BLNK.You can calculate the concentration of BLNKin the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 166235901
• NCBI GeneID: 29760
• NCBI Accession #: NP_001107566.1
• NCBI GenBank Nucleotide #: NM_001114094.1
• UniProt Accession #: Q8WV28; O75498; O75499; Q2MD49
• Molecular Weight: 44,451 Da
• NCBI Official Full Name: B-cell linker protein isoform 2
• NCBI Official Synonym Full Names: B-cell linker
• NCBI Official Symbol: BLNK
• NCBI Official Synonym Symbols: bca; AGM4; BASH; LY57; SLP65; BLNK-S; SLP-65
• NCBI Protein Information: B-cell linker protein; B cell adaptor containing SH2 domain; B cell linker protein; B-cell activation; B-cell adapter containing a SH2 domain protein; B-cell adapter containing a Src homology 2 domain protein; Src homology 2 domain-containing leukocyte protein of 65 kDa; cytoplasmic adapter protein
• UniProt Protein Name: B-cell linker protein
• Protein Family: B-cell linker protein
• UniProt Gene Name: BLNK
• UniProt Synonym Gene Names: BASH; SLP65; SLP-65
• UniProt Entry Name: BLNK_HUMAN

NCBI Description

This gene encodes a cytoplasmic linker or adaptor protein that plays a critical role in B cell development. This protein bridges B cell receptor-associated kinase activation with downstream signaling pathways, thereby affecting various biological functions. The phosphorylation of five tyrosine residues is necessary for this protein to nucleate distinct signaling effectors following B cell receptor activation. Mutations in this gene cause hypoglobulinemia and absent B cells, a disease in which the pro- to pre-B-cell transition is developmentally blocked. Deficiency in this protein has also been shown in some cases of pre-B acute lymphoblastic leukemia. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, May 2012]

Uniprot Description

BLNK: an adaptor protein that bridges the B-cell receptor-associated kinases (BCR) with a multitude of signaling pathways, regulating biologic outcomes of B-cell function and development. Plays an important role in BCR-mediated PLCG1 activation and Ca(2 ) mobilization. Does not seem to be not required for pre-BCR-mediated activation of MAP kinase and phosphatidyl-inositol 3 (PI3) kinase signaling. Plays a critical role in orchestrating the pro-B cell to pre-B cell transition. Following BCR activation, phosphorylated on tyrosine residues by SYK and LYN. When phosphorylated, serves as a scaffold to assemble downstream targets of antigen activation, including PLCG1, VAV1, GRB2 and NCK1. Its phosphorylation is required for both Ca(2 ) and MAPK signaling pathways. Defects in BLNK are the cause of hypoglobulinemia and absent B-cells.It has tumor supressor activity that is lost in many cases of childhood pre-B acute lymphoblastic leukemia (ALL). Two alternatively spliced isoforms have been described; these are differentially involved in activation and apoptosis of B lymphocytes.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 10q23.2-q23.33

Cellular Component: plasma membrane; intracellular; cytosol

Molecular Function: protein binding; SH3/SH2 adaptor activity; transmembrane receptor protein tyrosine kinase adaptor protein activity

Biological Process: positive regulation of signal transduction; B cell differentiation; inflammatory response; humoral immune response; transmembrane receptor protein tyrosine kinase signaling pathway

Disease: Agammaglobulinemia 4, Autosomal Recessive