Typical Testing Data/Standard Curve (for reference only)
Human beta parvin (PARVB) ELISA Kit | PARVB elisa kit
Human beta parvin (PARVB) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: PARVB ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-PARVB antibody and an PARVB-HRP conjugate. The assay sample and buffer are incubated together with PARVB-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PARVB concentration since PARVB from samples and PARVB-HRP conjugate compete for the anti-PARVB antibody binding site. Since the number of sites is limited, as more sites are occupied by PARVB from the sample, fewer sites are left to bind PARVB-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PARVB concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the parvin family of actin-binding proteins, which play a role in cytoskeleton organization and cell adhesion. These proteins are associated with focal contacts and contain calponin homology domains that bind to actin filaments. This family member binds to alphaPIX and alpha-actinin, and it can inhibit the activity of integrin-linked kinase. This protein also functions in tumor suppression. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Aug 2011]
Uniprot Description
PARVB: Probably plays a role in the regulation of cell adhesion and cytoskeleton organization. Belongs to the parvin family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Motility/polarity/chemotaxis; Actin-binding
Chromosomal Location of Human Ortholog: 22q13.2-q13.33
Cellular Component: cytoskeleton; focal adhesion; lamellipodium; plasma membrane; cytosol; Z disc
Molecular Function: protein binding; actin binding
Biological Process: lamellipodium biogenesis; actin cytoskeleton reorganization; cell projection biogenesis; cell adhesion
Research Articles on PARVB
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Product Notes
The Human PARVB parvb (Catalog #AAA7211075) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7211075 ELISA Kit recognizes Human PARVB. It is sometimes possible for the material contained within the vial of "beta parvin (PARVB), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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